中国药业2024，Vol.33Issue(2)：55-60,6.DOI:10.3969/j.issn.1006-4931.2024.02.013
失笑散化学成分分析
Analysis of Chemical Constituents in Shixiao Powder
摘要
Abstract
Objective To analyze the chemical constituents of Shixiao Powder.Methods Ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was adopted.The chromatographic column was Cortecs UPLC T3 column(100 mm×2.1 mm,1.6 μm),the mobile phase was 0.1%formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 0.3 mL/min,the column temperature was 30℃,the injection volume was 2 μL,and the electro-spray ionization(ESI)was adopted with the positive and negative ion modes.Results A total of 53 chemical constituents were obtained,including 23 in Typhae Pollen,14 in Faeces Trogopteror,and 16 were common in the two Chinese medicinal materials.The characteristic constituents of Typhae Pollen and the common constituents of the two Chinese medicinal materials were mainly flavonoids,while the characteristic constituents of Faeces Trogopteror were mainly terpenoids.Conclusion This method is easy and repeatable,which can provide a basis for the further research of the effect-material basis of Shixiao Powder.关键词
失笑散/蒲黄/五灵脂/化学成分/超高效液相色谱串联三重四极杆飞行时间质谱法Key words
Shixiao Powder/Typhae Pollen/Faeces Trogopteror/chemical constituent/ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry分类
医药卫生引用本文复制引用
王清,魏星,陈佩东,曹雨诞..失笑散化学成分分析[J].中国药业,2024,33(2):55-60,6.基金项目
国家自然科学基金[81873001]. （）
