中国真菌学杂志2023，Vol.18Issue(5)：397-400,4.
大鼠脑隐球菌感染模型建立及MRI评估
Establishment and MRI assessment of brain Cryptococcus infection rats model
摘要
Abstract
Objective To establish rats model of brain Cryptococcus infection and exploring the MRI imaging findings.Methods Thirty SD rats were injected with 10 μL of Cryptococcus neoformans suspension at a concentration of 1×106 CFU/mL using stereotaxic techniques.After then,T1WI,T2WI sequence scannings were performed on days 14,21,and 28.MRI was used to dynamically monitor the infection situation in rats brain,pathological dissection of the rats brain was used to observe tissue morphology.Results The success rate of the cryptococcal infection rats model established with a con-centration of 1×106 CFU/mL of C.neoformans suspension was 73.33%,with 60%cryptococcal granuloma and 13.33%cryptococcal meningitis.Other 20%rats showed no significant abnormal changes.The brain cryptococcal granuloma in rats showed round or nodular abnormal signal shadow in the caudate nucleus on MRI,low signal on T1WI and high signal on T2WI.MRI manifestations of meningitis include obvious linear abnormal signals in the meninges and ependyma.Conclusion The rats brain C.neoformans infection model was established with a concentration of 1×106 CFU/mL of C.neoformans suspension,which took a shorter time and had a higher success rate of inoculation.The MRI findings were highly consistent with pathological results,providing a good research model for the diagnosis and effectiveness evaluation of human brain cryp-tococcal infection.关键词
隐球菌/动物模型/MRI评估Key words
Cryptococcus/animal model/MRI assessment分类
医药卫生引用本文复制引用
李锦,王云玲,王俭,努尔比耶姆·阿布力克木,王刚,李嫣然,刘露露,姜春晖,王晓东,姜涛,和清源..大鼠脑隐球菌感染模型建立及MRI评估[J].中国真菌学杂志,2023,18(5):397-400,4.基金项目
国家自然科学基金新疆联合基金(U1903121) （U1903121）
