中国真菌学杂志2023，Vol.18Issue(5)：411-415,422,6.
外周血宏基因组二代测序诊断侵袭性肺曲霉病的运用
Application of peripheral blood metagenomic next generation sequencing in the diagnosis of invasive pulmo-nary aspergillosis
摘要
Abstract
Objective Metagenomic next generation sequencing was used to identify invasive pulmonary aspergillosis,which proved its important role in the clinical diagnosis of invasive pulmonary aspergillosis.Methods Three cases of inva-sive pulmonary aspergillosis were identified and diagnosed by peripheral blood metagenomic next generation sequencing in the Department of critical care medicine.The feasibility of peripheral blood metagenomic next generation sequencing in the diag-nosis of invasive pulmonary aspergillosis was analyzed and summarized.Results Of the three cases,case 1 had no underly-ing disease,case 2 had chronic obstructive pulmonary disease,and case 3 had T-lymphoblastic lymphoma/leukemia with a-granulocytosis after chemotherapy.Invasive pulmonary aspergillosis was diagnosed by bronchoalveolar lavage fluid(BALF)culture,sputum culture,peripheral blood metagenomic next generation sequencing,blood G test,GM test and other meth-ods.Conclusions Detection of Aspergillus cfDNA by peripheral blood metagenomic next generation sequencing,which is a rapid,and simple diagnostic method,can be used for the diagnosis of invasive pulmonary aspergillosis.关键词
侵袭性肺曲霉病/诊断/外周血/宏基因组二代测序Key words
invasive pulmonary aspergillosis/diagnosis/peripheral blood/metagenomic next generation sequencing分类
医药卫生引用本文复制引用
丁志荣,曾海文,林天来,陈伟文..外周血宏基因组二代测序诊断侵袭性肺曲霉病的运用[J].中国真菌学杂志,2023,18(5):411-415,422,6.基金项目
泉州市医疗卫生领域科技计划项目(2023NS037) （2023NS037）
