中国肿瘤生物治疗杂志2024，Vol.31Issue(1)：19-26,8.DOI:10.3872/j.issn.1007-385x.2024.01.003
IL-22/IL-22RA1通路在口腔鳞状细胞癌恶性进展中的作用及其机制
Role of IL-22/IL-22RA1 pathway in the malignant progression of oral squamous carcinoma and its mechanism
摘要
Abstract
Objective:To investigate the role of IL-22/IL-22RA1(IL-22 receptor A1)pathway in the malignant progression of oral squamous cell carcinoma(OSCC)and the underlying mechanisms.Methods:The expression levels of IL-22RA1 in OSCC tissues and paired para-cancerous tissues were analyzed using the GEO database and immunohistochemistry.The association between IL-22RA1 expression and the clinicopathological characteristics of OSCC patients was detected and analyzed by tissue microarray immunohistochemistry.The relationship between IL-22RA1 expression and the prognosis of OSCC patients was analyzed using the EBI ArrayExpress database.The expression of IL-22 and IL-22RA1 in OSCC tissues was examined by immunofluorescence,and their association was also analyzed.IL-22RA1 expression was knocked down in OSCC WSU-HN4 and CAL27 cells by RNA interference technology,and the efficiency was verified using qPCR.The effects of IL-22 on the colonogenesis and migration of OSCC cells in the negative control(siNC)group and IL-22RA1 knock-down(siIL-22RA1)group were determined by plate cloning and transwell assays,respectively.The effects of IL-22 on the expression of IL-22RA1 and the phosphorylation level of STAT1,STAT3 and ERK1/2 in OSCC cells were detected using the WB assay.Results:The mRNA expression of IL-22RA1 in OSCC tissues was significantly higher than that in para-cancerous tissues(P<0.05).The expression of IL-22RA1 was associated with tumor size(P<0.05),lymph node metastasis(P<0.01),and poor prognosis(P<0.05)of OSCC patients.There was no significant correlation between the expression levels of IL-22 and IL-22RA1 in OSCC tissues,and IL-22 did not affect on the expression level of IL-22RA1 in OSCC cells(all P>0.05).IL-22 significantly enhanced the colony formation and migration abilities(all P<0.01)of OSCC cells,and activated STAT1,STAT3 and ERK1/2 signals in OSCC cells.After knocking down IL-22RA1 in OSCC cells,IL-22 could not exert the above effects.Conclusion:IL-22/IL-22RA1 can promote the growth and metastasis of OSCC by regulating cell proliferation and migration,and the downstream mechanism may be the activation of ERK1/2-STAT1/3 signaling pathway.关键词
IL-22/IL-22受体A1/口腔鳞状细胞癌/WSU-HN4细胞/CAL27细胞/增殖/迁移Key words
IL-22/IL-22RA1/oral squamous cell carcinoma(OSCC)/WSU-HN4 cell/CAL27 cell/proliferation/migration分类
医药卫生引用本文复制引用
张晗,甘桂芳..IL-22/IL-22RA1通路在口腔鳞状细胞癌恶性进展中的作用及其机制[J].中国肿瘤生物治疗杂志,2024,31(1):19-26,8.基金项目
国家自然科学基金青年项目(No.82203551) （No.82203551）
上海市"医苑新星"青年医学人才培养资助计划(No.沪卫人事[2020]087号) （No.沪卫人事[2020]087号）
上海交通大学医工交叉项目(No.YG2022QN065) （No.YG2022QN065）
