溶瘤新城疫病毒抑制IL-6诱导的人胶质母细胞瘤U87MG细胞的迁移和侵袭OACSTPCD

Objective:To investigate the effect of oncolytic Newcastle disease virus(NDV)on the proliferation,migration and invasion of human glioblastoma U87MG cells induced by IL-6 and its possible mechanism.Methods:U87MG cells were divided into control group,IL-6 group,NDV group and NDV+IL-6 group.The cells in IL-6 group and NDV+IL-6 group were pretreated with 75 ng/mL IL-6 for 1 h,and the other groups were pretreated with DMEM for 1 h;then,the four groups were treated with DMEM,75 ng/mL IL-6,1 Hu NDV,1 Hu NDV+75 ng/mL IL-6 for 24 h,respectively.MTT assay,cell scratch test and Transwell invasion test were used to detect the effects of NDV and IL-6 on the proliferation,migration and invasion of U87MG cells,respectively.The protein expression levels of JAK2,p-JAK2,STAT3,p-STAT3 and MMP2 were detected by WB method.Results:Compared with the control group,the migration rate and number of invasive cells in IL-6 group were significantly increased(P<0.05 or P<0.01).Compared with IL-6 group,the proliferation rate of U87MG cells in NDV+IL-6 group was significantly decreased(P<0.05),and the migration rate and the number of invasive cells were significantly decreased(all P<0.01).WB results showed that compared with the control group,the p-STAT3/STAT3 ratio in IL-6 group was significantly increased(P<0.01),while the p-JAK2/JAK2 ratio,p-STAT3/STAT3 ratio and MMP-2 protein in NDV group were significantly decreased(P<0.05 or P<0.01);compared with IL-6 group,the p-STAT3/STAT3 ratio and the protein expression of MMP-2 in NDV+IL-6 group were significantly decreased(all P<0.05).Conclusion:NDV can inhibit IL-6 induced migration and invasion of human glioblastoma U87MG cells,and its mechanism may be related to its regulation of JAK2/STAT3 signaling pathway.