中国肿瘤生物治疗杂志2024，Vol.31Issue(1)：27-31,5.DOI:10.3872/j.issn.1007-385x.2024.01.004
溶瘤新城疫病毒抑制IL-6诱导的人胶质母细胞瘤U87MG细胞的迁移和侵袭
Oncolytic Newcastle disease virus inhibits IL-6 induced migration and invasion of human glioblastoma U87MG cells
摘要
Abstract
Objective:To investigate the effect of oncolytic Newcastle disease virus(NDV)on the proliferation,migration and invasion of human glioblastoma U87MG cells induced by IL-6 and its possible mechanism.Methods:U87MG cells were divided into control group,IL-6 group,NDV group and NDV+IL-6 group.The cells in IL-6 group and NDV+IL-6 group were pretreated with 75 ng/mL IL-6 for 1 h,and the other groups were pretreated with DMEM for 1 h;then,the four groups were treated with DMEM,75 ng/mL IL-6,1 Hu NDV,1 Hu NDV+75 ng/mL IL-6 for 24 h,respectively.MTT assay,cell scratch test and Transwell invasion test were used to detect the effects of NDV and IL-6 on the proliferation,migration and invasion of U87MG cells,respectively.The protein expression levels of JAK2,p-JAK2,STAT3,p-STAT3 and MMP2 were detected by WB method.Results:Compared with the control group,the migration rate and number of invasive cells in IL-6 group were significantly increased(P<0.05 or P<0.01).Compared with IL-6 group,the proliferation rate of U87MG cells in NDV+IL-6 group was significantly decreased(P<0.05),and the migration rate and the number of invasive cells were significantly decreased(all P<0.01).WB results showed that compared with the control group,the p-STAT3/STAT3 ratio in IL-6 group was significantly increased(P<0.01),while the p-JAK2/JAK2 ratio,p-STAT3/STAT3 ratio and MMP-2 protein in NDV group were significantly decreased(P<0.05 or P<0.01);compared with IL-6 group,the p-STAT3/STAT3 ratio and the protein expression of MMP-2 in NDV+IL-6 group were significantly decreased(all P<0.05).Conclusion:NDV can inhibit IL-6 induced migration and invasion of human glioblastoma U87MG cells,and its mechanism may be related to its regulation of JAK2/STAT3 signaling pathway.关键词
新城疫病毒/IL-6/胶质母细胞瘤/U87MG细胞/增殖/迁移/侵袭/JAK2/STAT3信号通路Key words
Newcastle disease virus/IL-6/glioblastoma/U87MG cell/proliferation/migration/invasion/JAK2/STAT3 signaling pathway分类
医药卫生引用本文复制引用
陶薇伊,秦莹,吴醒,郑婷婷,樊晓晖,梁莹..溶瘤新城疫病毒抑制IL-6诱导的人胶质母细胞瘤U87MG细胞的迁移和侵袭[J].中国肿瘤生物治疗杂志,2024,31(1):27-31,5.基金项目
国家自然科学基金(No.819605111) （No.819605111）
广西自然科学基金(No.2023GXNSFAA026280,No.2018GXNSFDA281043) （No.2023GXNSFAA026280,No.2018GXNSFDA281043）
