中国肿瘤生物治疗杂志2024，Vol.31Issue(1)：47-53,7.DOI:10.3872/j.issn.1007-385x.2024.01.007
LMAN2在HR阳性乳腺癌组织中的表达与患者预后的关系及其对MCF-7细胞增殖和迁移的影响
Relationship between LMAN2 expression in HR-positive breast cancer tissues and patient prognosis and its effect on proliferation and migration of MCF-7 cells
摘要
Abstract
Objective:To explore the relationship between the expression level of mannose-binding lectin 2(LMAN2)in hormone receptor(HR)-positive breast cancer tissues and patient prognosis as well as its effects on the proliferation and migration of MCF-7 cells.Methods:The differential expression of LMAN2 in breast cancer tissues and normal breast tissues as well as its relationship with patients'prognosis were analyzed by TCGA,Bc-GenExMiner,GEPIA and Kaplan-Meier Plotter databases.si-LMAN2#1,si-LMAN2#2 and si-NC were transfected into MCF-7 cells using small RNA interference technology,and the LMAN overexpression vector(pc-LMAN)and empty vector pcDNA3.1 negative control(pc-NC)were transfected into MCF-7 cells;and accordingly,the experimental groups were named si-LMAN2#1 group,si-LMAN2#2 group,si-NC group,pc-LMAN2 group and pc-NC group.The mRNA and protein expression levels of LMAN2 in MCF-7 cells of each group were detected by qPCR and WB assay,respectively.The effects of knockdown and overexpression of the LMAN2 on the proliferation,colony formation and migration as well as the expression of AKT signaling pathway-related proteins in MCF-7 cells were detected using CCK-8,colony formation,Transwell migration and WB assay,etc.Results:The expression level of LMAN2 in breast cancer tissues was significantly higher than that in normal breast tissues(P<0.001),and the expression of LMAN2 in HR-positive breast cancer tissues was significantly higher than that in HR-negative breast cancer tissues(P<0.001).In contrast to low LMAN2 expression,high expression of LMAN2 was associated with poor prognosis in HR-positive breast cancer patients.Knockdown of LMAN2 significantly inhibited the proliferation and migration abilities of MCF-7 cells(P<0.01 or P<0.001),while overexpression of LMAN2 significantly enhanced the proliferation and migration abilities of MCF-7 cells(all P<0.001).The protein expression levels of PTEN and P21 in MCF-7 cells with LMAN2 knockdown were significantly elevated,while the protein expression of p-AKT was significantly decreased(all P<0.01).Conclusion:LMAN2 is highly expressed in breast cancer tissues and HR-positive breast cancer tissues and is associated with poor prognosis.The high expression of LMAN2 is associated with the proliferation and migration of MCF-7 cells,and the mechanism may involve the AKT signaling pathway.关键词
甘露糖结合凝集素2/激素受体阳性/乳腺癌/MCF-7细胞/增殖/迁移/AKT信号通路Key words
mannose-binding lectin 2(LMAN2)/hormone receptor(HR)-positive/breast cancer/MCF-7 cell/proliferation/migration/AKT signaling pathway分类
医药卫生引用本文复制引用
延常姣,黄美玲,肖晶晶,凌瑞..LMAN2在HR阳性乳腺癌组织中的表达与患者预后的关系及其对MCF-7细胞增殖和迁移的影响[J].中国肿瘤生物治疗杂志,2024,31(1):47-53,7.基金项目
国家自然科学基金(No.81572917) （No.81572917）
陕西省重点研发计划(No.2022ZDLSF02-07) （No.2022ZDLSF02-07）
陕西省自然科学基础研究计划(No.2023-JC-QN-0965) （No.2023-JC-QN-0965）
