中国中医药信息杂志2024，Vol.31Issue(2)：123-129,7.DOI:10.19879/j.cnki.1005-5304.202304297
化痰祛瘀汤对脑小血管病大鼠认知功能及脑组织GABA、VILIP-1表达的影响
Effects of Huatan Quyu Decoction on Cognitive Function and Expressions of GABA and VILIP-1 in Brain Tissue in Rats with Cerebral Small Vessel Disease
摘要
Abstract
Objective To observe the effects of Huatan Quyu Decoction on cognitive function and the expressions of GABA and VILIP-1 in brain tissue of rats with cerebral small vessel disease;To discuss its mechanism for treatment on cerebral small vessel disease.Methods Totally 48 male SD rats were randomly divided into blank group,model group,Huatan Quyu Decoction low-and high-dosage groups,with 12 rats in each group.Except for the blank group,a rat model of cerebral small vessel disease was prepared by in vitro injection of homologous microemboli.Huatan Quyu Decoction low-and high-dosage groups were given Huatan Quyu Decoction 1.25 and 2.5 g/kg by gavage,the blank group and model group were gavage with equal amounts of distilled water for 28 consecutive days.Morris water maze experiment was conducted on day 1,7,14,and 28 after administration to evaluate the learning and memory abilities of rats,HE staining was used to observe pathological changes in hippocampal tissue,and immunohistochemical staining was used to detect the expressions of GABA and VILIP-1 proteins in brain tissue.Results Compared with the blank group,the escape latency of Morris water maze experiment in model group significantly prolonged(P<0.05),and the number of crossing platforms was significantly reduced(P<0.05);the arrangement of hippocampal tissue cells was disordered,gaps widen,and nuclei atrophy and necrosis,the GABA expression in brain tissue significantly decreased(P<0.05),while the VILIP-1 expression significantly increased(P<0.05).Compared with the model group,the escape latency of Morris water maze experiment in the Huatan Quyu Decoction low-and high-dosage groups significantly shortened(P<0.05)on day 7,14,and 28 of administration,and the number of crossing platforms significantly increased(P<0.05),GABA expression significantly increased(P<0.05),while VILIP-1 expression significantly decreased(P<0.05).Compared with the Huatan Quyu Decoction low-dosage group,the escape latency of Morris water maze experiment in Huatan Quyu Decoction high-dosage group decreased at various time points,and the number of crossing platforms increase,the pathological damage of hippocampal tissue was reduced,the expression of GABA in brain tissue increased,and the expression of VILIP-1 decreased,with statistical significance(P<0.05).Conclusion Huatan Quyu Decoction can increase the expression of GABA in brain tissue and inhibit the expression of VILIP-1,thereby improve the cognitive function of rats with cerebrovascular disease.关键词
脑小血管病/认知障碍/γ-氨基丁酸/视锥蛋白样蛋白1/大鼠Key words
cerebral small vessel disease/cognitive impairment/GABA/VILIP-1/rats分类
医药卫生引用本文复制引用
田与乾,方永军,胡亚莉,张慧,黄晓峰,韦鹏方,赵鑫雅,闫咏梅..化痰祛瘀汤对脑小血管病大鼠认知功能及脑组织GABA、VILIP-1表达的影响[J].中国中医药信息杂志,2024,31(2):123-129,7.基金项目
国家自然科学基金(81973843) （81973843）
陕西省重点研发计划(2023-YBSF-435) （2023-YBSF-435）
中国民族医药学会科研项目(2020ZY129-410401) （2020ZY129-410401）
陕西省创新人才推进计划-重点科技创新团队计划(2017KCT-26) （2017KCT-26）
国家中医药管理局西学中骨干人才培训项目(2019年) （2019年）
