中国中医药信息杂志2024，Vol.31Issue(2)：144-148,5.DOI:10.19879/j.cnki.1005-5304.202307085
玫瑰花HPLC指纹图谱研究
Study on HPLC Fingerprint of Rosae Rugosae Flos
摘要
Abstract
Objective To establish the HPLC fingerprint of Rosae Rugosae Flos;To provide references for the quality evaluation of Rosae Rugosae Flos.Methods The HPLC analysis was carried on a COSMOSIL 5C18-MS-Ⅱ column(4.6 mm×250 mm,5 μm);the mobile phase was 2.5 % acetonitrile + 0.1 % formic acid aqueous solution(A)-acetonitrile + 0.1 % formic acid(B)with gradient elution at the flow rate of 0.5 Ml/min;the column temperature was 40℃;the detection wavelength was 350 nm.The similarity of 13 batches of samples was evaluated by Similarity Evaluation System for Chromatographic Fingerprint of TCM(2012 edition).Qualitative analysis was carried out by LC-MS technology.The overall quality evaluation of Rosae Rugosae Flos was carried out by combining clustering analysis,principal component analysis and orthogonal partial least square discrimination.Results The common mode of HPLC fingerprints of Rosae Rugosae Flos was established,and the similarity of 13 samples was good.9 compounds were identified preliminary.13 batches of samples were aggregated into 3 categories by chemical pattern recognition.Conclusion The fingerprints of Rosae Rugosae Flos established in this study combines with chemical pattern recognition method,which has high sensitivity and strong specificity,can provide a basis for the quality evaluation of Rosae Rugosae Flos.关键词
玫瑰花/指纹图谱/液相色谱-质谱法/相似度/化学模式识别Key words
Rosae Rugosae Flos/fingerprint/LC-MS/similarity/chemical pattern recognition分类
医药卫生引用本文复制引用
齐和日玛,布仁,成日青,王帅,何琴芳,塔娜,萨仁高娃..玫瑰花HPLC指纹图谱研究[J].中国中医药信息杂志,2024,31(2):144-148,5.基金项目
国家自然科学基金(82060769) （82060769）
内蒙古自治区自然科学基金(2020MS08129) （2020MS08129）
内蒙古医科大学善学人才培养计划(ZY0201030) （ZY0201030）
