神经药理学报2023，Vol.13Issue(4)：19-28,10.DOI:10.3969/j.issn.2095-1396.2023.04.004
某复方利多卡因乳膏对小鼠局部表面麻醉起效时间的评估
Evaluation of the Onset of Action Time of A Compound Lidocaine Cream on Local Surface Anesthesia in Mice
聂永胜 1陈曦 1雷莹 1梁萍萍 1冉茜 1吴谦 1林心文 1曾琳 1王有柱 1周泉1
作者信息
- 1. 冠科生物技术(中山)有限公司,中山,528400,中国
- 折叠
摘要
Abstract
Objective:To investigate the onset time of a compound lidocaine cream on topical anesthesia in mice.Methods:Five strains of mice commonly used in medical experi-ments(BALA-C/Nude,BALB/C,NOD/SCID,C57BL/6J,C3H)were selected.Compound li-docaine cream was applied on the back respectively,and the acupuncture operation simulated inoculating PDX tumor under the skin was used,and then the expression change,acupunc-ture evasion response and needle pain response of mice were evaluated by scoring method,and the smaller the response,the lower the score,indicating that the anesthesia effect was better.The time points were:0,10,20,30,40,50,60 min.Results:The response to local anes-thesia was different in mice of different strains,but the general trend was as follows:after 40 minutes,the score was generally reduced,indicating that the efficacy of local anesthetic drugs played a role.Conclusion:In mice undergoing PDX tumor mass implantation,it is rec-ommended to start the operation after 40 min after applying compound lidocaine when sim-ple dermatotomy and suture,which is more in line with animal welfare.关键词
复方利多卡因/局麻效果/人源肿瘤异种移植Key words
compound lidocaine/local anesthesia effect/patient-derived tumor xenograft分类
医药卫生引用本文复制引用
聂永胜,陈曦,雷莹,梁萍萍,冉茜,吴谦,林心文,曾琳,王有柱,周泉..某复方利多卡因乳膏对小鼠局部表面麻醉起效时间的评估[J].神经药理学报,2023,13(4):19-28,10.
