植物营养与肥料学报2023，Vol.29Issue(12)：2193-2207,15.DOI:10.11674/zwyf.2023197
有机肥对煤矿复垦土壤团聚体碳氮磷含量及细菌群落的影响
Effects of manures on carbon,nitrogen,phosphorus content and bacterial community in reclaimed soil aggregates
摘要
Abstract
[Objectives]Applying manures can effectively improve the fertility,aggregates number and microbial activities of reclaimed soils.We investigated the effects of different manures on the carbon,nitrogen and phosphorus contents and bacteria communities in the aggregates of a reclaimed soil.[Methods]A field experiment was carried out in the coal mining subsidence area of Xiaoyi City,Shanxi Province for 5 consecutive years.The experiment treatments contained blank control,chicken manure,pig manure,cow manure,and chemical fertilizer(CF).The total annual nutrient inputs were N 200 kg/hm2,P2O5100 kg/hm2,and K2O 100 kg/hm2 for all the four fertilizer treatments,the manure application amount was calculated by P2O5100 kg/hm2,the gaps for N and K2O were supplemented with chemical fertilizers.After maize harvest on the fifth year,0-20 cm soil samples were collected,and divided into three particle sizes(>2 mm,0.25-2 mm and<0.25 mm)using dry screening method.The soil organic carbon(SOC),dissolved organic carbon(DOC),total nitrogen(TN),total phosphorous(TP),available phosphorous(AP),microbial biomass carbon,nitrogen and phosphorous(SMBC,SMBN and SMBP)in each particle size were analysed using conventional methods,and the relative abundance of bacterial communities were calculated using 16S rRNA high throughput sequencing method.[Results]1)Compared to CK,CF significantly reduced,while manures increased the proportion of>2 mm aggregates,and the increment in chicken manure treatment reached significant level.In addition,manures significantly increased the proportion of>2 mm aggregates,compared to CF,the increment in chicken,pig,and cow manure treatments were 35.56％,22.32％and 20.25％,respectively.Fertilization substantially elevated the C,N,and P contents in all the three particle sizes of aggregates,manures exhibited more pronounced increment than CF,with increase of SOC 50％,TN 15％,TP 43％on average,respectively.In>2 mm aggregates,manures resulted in 42％higher DOC than CF,and pig manure resulted in 28％and 40％higher DOC than chicken and cow manures;in 0.25-2 mm aggregates,manure treatments had 41％,73％,42％and 260％higher TN,TP,SMBN,and SMBP than CF,respectively,and the increment of above indexes were in order of chicken manure>pig manure≥cow manure.In<0.25 mm aggregates,the increments of manures over CF were by 75％in organic C,373％in AP and 124％in SMBC,respectively.Among the manures,the improvement of TN,TP,SMBN and SMBP by fertilization all showed the order of chicken manure>pig manure≥cow manure≥chemical fertilizer,while the improvement of SOC,DOC and SMBC contents were the most significant in pig manure.2)Actinobacteria,Proteobacteria,Chloroflexi,Acidobacteria and Bacteroidetes were the dominant phyla in soil.Fertilization significantly increased the bacterial diversity and richness in 0.25-2 mm aggregates.Compared to CK,fertilization reduced the relative abundance of Acidobacteria by 17.8％-110％,increased that of Proteobacteria by 8.98％-43.5％,and Bacteroidetes by 19.9％-56.0％,and the increment by manures reached significant levels.PICRUSt function prediction showed that manures significantly increased the abundance of functional genes related to C,N and P metabolism,with the greatest elevation in chicken manure treatment.[Conclusions]Manures application could improve the stability of reclaimed soil structure,and increase carbon,nitrogen and phosphorus contents in aggregates.The effects are varied among manures,pig manure is the most effective in carbon content,and chicken manure is in phosphorus content,bacterial diversity and abundance.关键词
有机肥/复垦土壤/团聚体/碳/氮/磷/PICRUSt功能预测/细菌群落Key words
manure/reclaimed soil/aggregate/carbon/nitrogen/phosphorus/PICRUSt functional prediction/bacterial community引用本文复制引用
孙晓东,栗海鹏,高文俊,张杰,徐明岗,郝鲜俊..有机肥对煤矿复垦土壤团聚体碳氮磷含量及细菌群落的影响[J].植物营养与肥料学报,2023,29(12):2193-2207,15.基金项目
国家自然科学基金项目(41601327) （41601327）
山西省基础研究计划项目(20210302123414) （20210302123414）
山西省重点研发计划项目(202102140601010-5) （202102140601010-5）
山西农业大学科技创新基金项目(2016ZZ14) （2016ZZ14）
山西省回国留学人员科研资助项目(2023-090). （2023-090）
