遵义医科大学学报2024，Vol.47Issue(1)：18-24,7.
转谷氨酰胺酶交联对溶菌酶潜在致敏性的影响
Effect of transglutaminase catalyzed crosslinking on the potential allergenicity of lysozyme
摘要
Abstract
Objective To explore the effect of transglutaminase catalyzed cross-linking on the IgG and IgE bind-ing ability of egg allergen lysozyme,so as to provide theoretical basis and data support for the processing of hypo-allergenic egg products.Methods First,ion exchange chromatography was used to separate and prepare pure ly-sozyme.After co-incubation of lysozyme with transglutaminase,its cross-linking was observed by SDS-PAGE,the change in the IgG binding capacity of the cross-linked product was verified by using rabbit anti-lysozyme spe-cific serum,and the IgE binding capacity of the cross-linked product was verified by using the sera of egg allergy patients.At the same time,the optimal reaction conditions for cross-linking to reduce the allergenicity of lyso-zyme were determined.Results Transglutaminase could catalyze the cross-linking reaction of lysozyme,and the IgG binding ability of lysozyme after cross-linking was significantly reduced,but its IgE binding ability only changed at transglutaminase/substrate of 10 U/g,and at reaction time of 6 h.The lowest IgE binding capacity reduced about 32％.Conclusion Transglutaminase can catalyze the cross-linking reaction of egg allergen lyso-zyme,but the IgE binding ability of the cross-linked product was not reduced ideally.关键词
溶菌酶/转谷氨酰胺酶/致敏性Key words
lysozyme/transglutaminase/allergenicity分类
医药卫生引用本文复制引用
张晨晨,黎江东,周荣钦,麻小娟..转谷氨酰胺酶交联对溶菌酶潜在致敏性的影响[J].遵义医科大学学报,2024,47(1):18-24,7.基金项目
国家自然科学基金资助项目(NO:31660443) （NO:31660443）
遵义医科大学优秀青年人才项目(NO:18zy-009) （NO:18zy-009）
贵州省科技厅项目[NO:黔科合基础(2019)1348号]. （2019）
