遵义医科大学学报2024，Vol.47Issue(1)：53-61,9.
基于RNA-Seq技术初步探索顺铂在口腔鳞状细胞癌的相关耐药机制
Investigating cisplatin drug resistance mechanisms in oral squamous cells carcinoma through RNA-Seq technology:a preliminary study
摘要
Abstract
Objective Investigating the genes and signaling pathways related to cisplatin chemoresistance in oral squamous cell carcinoma(OSCC).Methods We established an in vitro model of cisplatin-induced resistance in an OSCC cell line.Using RNA sequencing,we compared the transcriptomic difference of cisplatin-resistant(HN6-R)and sensitive(HN6-WT_Control)cell lines.Differential expression analysis was conducted to identify key genes and pathways involved in chemoresistance.Gene set enrichment analysis(GSEA)was utilized to fur-ther elucidate the biological significance of these changes.Results Transcriptomic analysis revealed 216 differen-tially expressed genes(DEGs)in HN6-R cells,including 55 downregulated and 161 upregulated genes,relative to HN6-WT_Control cells(log2Fold Change＞1;adjusted P value＜0.05).GSEA analysis identified several critical pathways associated with chemoresistance,notably the IL6/JAK2/STAT3,TNF-α and NF-κB,and vari-ous immune response pathways.Prominent among the altered genes were CXCL10,CXCL11,NR4A3,and IG-FBP3.Conclusion Our findings highlight the upregulation of IL6/JAK2/STAT3 and other immune-related path-ways in cisplatin-resistant OSCC cells,suggesting their potential as biomarkers for chemotherapy response and as therapeutic targets.This study provides new insights into the molecular mechanisms of cisplatin resistance in OS-CC,offering a foundation for future research and therapeutic development.关键词
口腔鳞癌/顺铂/化疗/耐药/差异基因/生物信息学Key words
oral squamous cell carcinoma/cisplatin/chemotherapy/drug resistance/differentially expressed genes/bioinformatics分类
医药卫生引用本文复制引用
张万林,李一村,杨宏宇..基于RNA-Seq技术初步探索顺铂在口腔鳞状细胞癌的相关耐药机制[J].遵义医科大学学报,2024,47(1):53-61,9.基金项目
深圳市科技创新委员会基础研究重点项目(NO:JCYJ20220818102816036). （NO:JCYJ20220818102816036）
