中医药信息2024，Vol.41Issue(1)：6-11,17,7.DOI:10.19656/j.cnki.1002-2406.20240102
强志组方调控PI3K/Akt/TNF-α信号通路干预抽动障碍的实验研究
Experimental Study on the Intervention of Qiangzhi Formula in Tic Disorder by Regulating PI3K/Akt/TNF-α Signaling Pathway
摘要
Abstract
Objective:To explore the mechanism of Qiangzhi(QZ)Formula in the treatment of tic disorder(TD)based on PI3K/Akt/TNF-α signaling pathway.Methods:Forty-two SD rats were randomly divided into blank group,model group,tiapride group,and low-dose,medium-dose and high-dose QZ formula groups.The tic disorder model was established by intraperitoneal injection of iminodipropionitrile(IDPN).The low-dose,medium-dose and high-dose QZ formula groups were given 4.455 g/(kg·d),8.91 g/(kg·d)and 17.82 g/(kg·d)water decoction by gavage respectively.Tiapride group was given tiapride suspension 0.027 g/(kg·d)by gavage.The rats in the blank group and the model group were given the same dose of normal saline by gavage once a day for 28 consecutive days.The behavioral changes of rats were evaluated by stereotyped behavior score and motor behavior score.After the intervention,Western blot and qPCR were used to detect the mRNA and protein expression of PI3K,AKT and TNF-α in the striatum.The serum levels of IL-6 and TNF-α were detected by enzyme-linked reaction adsorption assay(ELISA).Results:After modeling,compared with the blank group,the rats in the model group showed abnormal motor behavior and stereotyped behavior.The protein expression levels of PI3K and TNF-α and the phosphorylation level of p-Akt/Akt increased in the striatum(P＜0.01,P＜0.01,P＜0.05);PI3K,Aktand mRNA expression levels increased(P＜0.001,P＜0.01,P＜0.05);and serum IL-6 and TNF-α levels increased(P＜0.001,P＜0.01).After 28 days of drug intervention,compared with the model group,the motor behavior score and stereotype-behavior score of tiapride group and AZ formula groups decreased(P＜0.001,P＜0.05).The expression levels of PI3K and TNF-α protein in striatum of QZ formula low-dose group decreased(P＜0.05).The expression levels of PI3K,Akt,TNF-α and p-Akt/Akt phosphorylation in the middle and high dose of QZ formula group decreased(P＜0.001,P＜0.05,P＜0.05).The expression levels of PI3K,Akt and mRNA in striatum of middle and high dose QZ formula group decreased(P＜0.01,P＜0.05).The level of serum IL-6 in tiapride group and QZ group decreased(P＜0.01,P＜0.001),and the serum TNF-α level decreased in each dose group of QZ formula(P＜0.01,P＜0.01,P＜0.05).Conclusions:Qiangzhi Formula can alleviate the abnormal motor behavior and stereotype behavior of rats with tic disorders,which may be related to the PI3K/Akt/TNF-α signaling pathway.关键词
强志组方/抽动障碍/PI3K/Akt/TNF-α信号通路Key words
Qiangzhi Formula/Tic disorder/PI3K/Akt/TNF-α signaling pathway引用本文复制引用
詹婷,姜韫赟,王妹,靳无菲,李婷,阎兆君..强志组方调控PI3K/Akt/TNF-α信号通路干预抽动障碍的实验研究[J].中医药信息,2024,41(1):6-11,17,7.基金项目
国家自然科学基金项目(81774249) （81774249）
志意辨证学术流派传承工作室项目(鲁卫函[2021]45号) （鲁卫函[2021]45号）
济南市"高校20条"资助项目(2020GXRC013) （2020GXRC013）
