Tb^(3+)-nucleic acid probe-based label-free and rapid detection of mercury pollution in foodOACSTPCD
Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.
Xuhan Xia;Chenxi Zhou;Yulin Zhu;Yi Dong;Qiang He;Mohammad Rizwan Khan;Yuanlong Chi;Rosa Busquets;Ruijie Deng;Yao Ren;
College of Biomass Science and Engineering,Healthy Food Evaluation Research Center,Sichuan University,Chengdu 610065,ChinaDepartment of Chemistry,College of Science,King Saud University,Riyadh 11451,Saudi ArabiaSchool of Life Sciences,Pharmacy and Chemistry,Kingston University London,Kingston Upon Thames KT12EE,UK
轻工业
Mercury pollutionFood safetyNucleic acid probeLabel-freeTerbium
《Food Science and Human Wellness》 2024 (002)
P.993-998 / 6
financially supported by National Natural Science Foundation of China(22074100);the Young Elite Scientist Sponsorship Program by CAST(YESS20200036);the Researchers Supporting Project Number RSP-2021/138;King Saud University,Riyadh,Saudi Arabia;Technological Innovation R&D Project of Chengdu City(2019-YF05-31702266-SN);Sichuan University-Panzhihua City joint Project(2020CDPZH-5)。
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