动物营养学报2023，Vol.35Issue(12)：7903-7919,17.DOI:10.12418/CJAN2023.717
固体饲料不同饲喂量对哺乳期牦牛犊牛胰腺发育的影响及代谢组学分析
Effects of Different Feeding Levels of Solid Feed on Pancreatic Development of Suckling Yak Calves and Its Metabolomic Analysis
摘要
Abstract
After the birth of a yak calf,the early adaptation of calves to solid feed is critical to their growth and digestive system development,as it involves the transition from liquid to solid feed and from a non-ruminant to a ruminant nutritional type.It is considered the focus of calf breeding.The aim of this experiment was to inves-tigate the effects of different feeding levels of solid feed on the development of pancreas in suckling yak calves using non-targeted metabolomics techniques.Twenty-one healthy yak calves(males)with similar age[(60± 3)days of age]and weight[(44.45±2.50)kg]were selected as test animals and randomly divided into three groups:the low feeding group(40％feed intake group,IR40 group),the medium feeding group(70％feed intake group,IR70 group)and the high feeding group(free feeding group,AL group),with 7 yak calves in each group.All three groups were fed equal amounts of milk replacer and different amounts of solid feed(con-centrate and alfalfa hay).The pretest period lasted for 30 days,and the test period lasted for 90 days.The re-sults showed as follows:1)the body weight,pancreatic organ index and total percentage of external secretory part area were significantly higher in the AL group than the IR70 group(P＜0.05);additionally,the body weight,pancreatic weight,pancreatic organ index and total percentage of external secretory part area were sig-nificantly higher in the AL group than the IR40 group(P＜0.05).2)Pancreatic amylase,pancreatic protease activity and insulin content were significantly higher in the AL group than the IR70 group(P＜0.05).Moreo-ver,pancreatic amylase,trypsin and chymotrypsin activities and glucagon,insulin and growth inhibitor con-tents were significantly higher in the AL group than the IR40 group(P＜0.05);glucagon content was signifi-cantly higher in the IR70 group than the IR40 group(P＜0.05).3)Metabolomic analysis revealed a significant increase in ergocalciferol content in steroid biosynthesis in the IR70 group compared with the AL group(P＜0.05);progesterone content was significantly increased in the oocyte meiotic pathway,progesterone-mediated oocyte maturation pathway and breast cancer pathway(P＜0.05);in the pyrimidine metabolic pathway,the contents of deoxycytidylic acid and uracil were significantly increased(P＜0.05);the content of UDP-N-acetylglucosamine in the insulin resistance pathway was significantly decreased in the IR70 group compared with the IR40 group(P＜0.05);the ergocalciferol content in the steroid biosynthesis pathway was significantly increased(P＜0.05);the testosterone content in the endocrine resistance pathway was significantly increased(P＜0.05);the O-phosphate-L-serine content in the glycine,serine and threonine metabolic pathways was sig-nificantly decreased(P＜0.05);UDP-N-acetylglucosamine content was significantly reduced in the neomycin,kanamycin and gentamicin biosynthetic pathways(P＜0.05);in the steroid hormone biosynthetic pathway,corticosterone and testosterone contents were significantly increased(P＜0.05).In summary,it is concluded that when suckling yak calves are bred by milk replacer feeding,increasing the amount of solid feed improve the quality of growth and development of yak calves,and promote the development of the morphology and function of their pancreatic tissues;and the medium feeding amount can induce the differentiation of pancreatic cells in suckling yak calves,and enhance the body's ability to digest and absorb small molecule nutrients.[Chi-nese Journal of Animal Nutrition,2023,35(12):7903-7919]关键词
哺乳期/牦牛犊牛/固体饲料饲喂量/胰腺发育/代谢组学Key words
suckling period/yak calves/feeding level of solid feed/pancreas development/metabolomics分类
农业科技引用本文复制引用
梁玉鸿,焦洋,刘书杰,杨得玉,周玉青,张万明,崔占鸿..固体饲料不同饲喂量对哺乳期牦牛犊牛胰腺发育的影响及代谢组学分析[J].动物营养学报,2023,35(12):7903-7919,17.基金项目
国家自然科学基金项目(32260853) （32260853）
青海省中央引导地方科技发展资金项目(2023-ZY-017) （2023-ZY-017）
