动物营养学报2023，Vol.35Issue(12)：7988-8000,13.DOI:10.12418/CJAN2023.724
饲粮中添加葡萄籽原花青素对热应激母兔繁殖性能及其仔兔生长性能的影响
Effects of Dietary Grape Seed Procyanidins on Reproductive Performance of Female Rabbits and Growth Performance of Offspring under Heat Stress
摘要
Abstract
The aim of this study was to investigate the effects of grape seed procyanidins(GSP)on reproduc-tive performance of female rabbits and growth performance of offspring under heat stress condition.Experiment 1:to study the effects of GSP supplementation during pregnancy on reproductive performance of female rabbits and growth performance of offspring under heat stress,two hundred and forty-eight primiparous female rabbits generated from Elco multiline cross system with similar body weight and condition were selected and randomly divided into 4 groups with 62 replicates per group and 1 rabbit per replicate.From the start of the trial until the delivery of female rabbits,female rabbits in the four groups were fed experimental diets which supplemented with 0(control group),200,300 and 400 mg/kg GSP based on the basal diet,respectively;from delivery on day 37 of the trial to weaning of offspring at 35 days of age,female rabbits in the four groups were fed the bas-al diet.The pre-experimental period lasted for 7 days,and the experimental period lasted for 72 days.Experi-ment 2:to investigate the effects of GSP supplementation in different stages of pregnancy on reproductive per-formance of female rabbits and growth performance of offspring under heat stress condition,one hundred and twenty-six primiparous female rabbits generated from Elco multiline cross system with similar body weight and condition were selected and randomly divided into 3 groups,they were control group,early gestation group and mid-late pregnancy group,with 42 replicates per group and 1 rabbit per replicate.Female rabbits in the early gestation group and mid-late pregnancy group were fed the experiment which supplemented with 300 mg/kg GSP based on the basal diet at days 1 to 12 of pregnancy and days 13 to 30 of pregnancy,respectively,and fed the basal diet at other time;female rabbits in the control group were fed the basal diet during the whole experi-ment period.The pre-experimental period lasted for 7 days,and the experimental period lasted for 72 days.The results of experiment 1 showed as follows:compared with the control group,the 300 mg/kg GSP supplementa-tion during pregnancy significantly increased the litter size,live litter size and litter weight born alive(P＜0.05),and significantly increased the No.of kits per litter and litter weight at 7 days age,the litter weight at 14 days age,and the No.of kits per litter and litter weight at 21 days age of offspring(P＜0.05);compared with the control group,the 300 mg/kg GSP supplementation during pregnancy significantly increased the activ-ities of superoxide dismutase(SOD)and catalase(CAT)in serum of female rabbits on the 6th day of estrus synchronization(P＜0.05),and significantly reduced the content of malondialdehyde(MDA)in serum(P＜0.05).In addition,at the 15th day of pregnancy,compared with the control group,the 400 mg/kg GSP sup-plementation during pregnancy significantly increased the content of progesterone in serum of female rabbits(P＜0.05),and the 200 mg/kg GSP supplementation during pregnancy significantly reduced the content of MDA in serum(P＜0.05),furthermore,the 300,400 mg/kg GSP supplementation during pregnancy signifi-cantly reduced the content of tumor necrosis factor-α(TNF-α)in serum(P＜0.05);there were no significant differences in the contents of interleukin-10(IL-10),immunoglobulin G(IgG),immunoglobulin A(IgA)and immunoglobulin M(IgM)in serum among the groups(P＞0.05).The results of experiment 2 showed as follows:compared with the control group,the 300 mg/kg GSP supplementation in the mid-late stage of preg-nancy significantly increased the live litter size,total litter weight,litter weight born alive and average weight of kits born alive(P＜0.05),and significantly increased the No.of kits per litter at 21 days of age and weaning(35 days of age),the litter weight at 14 days of age and weaning(P＜0.05).In summary,under heat stress condition,the supplementation of 300 mg/kg GSP in the diet can significantly improve the reproductive per-formance of female rabbits and the growth performance of offspring.Furthermore,the supplementation of 300 mg/kg GSP in the diet in mid-late stage of pregnancy(days 13 to 30 of pregnancy)showed similarly im-provement effect.[Chinese Journal of Animal Nutrition,2023,35(12):7988-8000]关键词
葡萄籽原花青素/热应激/母兔/繁殖性能/仔兔/生长性能Key words
grape seed procyanidins/heat stress/female rabbits/reproduction performance/young rabbits/growth performance分类
农业科技引用本文复制引用
李祺,梁慧,龙润泽,刘旭阳,吴英杰,刘宁,秦应和..饲粮中添加葡萄籽原花青素对热应激母兔繁殖性能及其仔兔生长性能的影响[J].动物营养学报,2023,35(12):7988-8000,13.基金项目
国家自然科学基金青年基金(32000082) （32000082）
现代农业产业技术体系建设专项资金资助(CARS-43) （CARS-43）
