动物营养学报2023，Vol.35Issue(12)：8133-8147,15.DOI:10.12418/CJAN2023.736
昆明犬源乳酸菌的分离、鉴定与益生特性研究
Isolation,Identification and Probiotic Characteristics of Lactic Acid Bacteria Derived from Kunming Dog
摘要
Abstract
This study aimed to isolate canine-derived lactic acid bacteria,which would provide a basis for the development of canine microecological preparations.Using fresh feces of Kunming dog as the source of strain i-solation,and lactic acid bacteria were isolated and purified using MRS agar supplemented with calcium carbon-ate.A total of 21 strains of lactic acid bacteria were identified based on 16S rRNA sequencing.Their antibiotic sensitivity,antagonistic activity,gastrointestinal tolerance,adhesion ability and anti-inflammatory ability were determined and evaluated.The probiotic characteristics between different strains were compared comprehensive-ly.The results showed that seven strains with good antibiotic sensitivity,bacteriostatic activity and gastrointesti-nal fluid tolerance were isolated and screened from the fresh feces of Kunming dogs,and MKLQ3807-13,MKLQ3807-35,MKLQ4704-19,MKLQ4704-30,MKLQ4704-49 and MKCH1884-30 were Lactobacillus re-uteri and MKCH1884-43 was Lactobacillus gasseri.MKLQ3807-13,MKLQ3807-35,MKLQ4704-19,MKLQ4704-30 and MKCH1884-43 had strong adhesion ability to intestinal epithelial cells HT-29.MKLQ3807-13 and MKCH1884-43 could significantly decrease the relative expression level of proinflammato-ry gene interleukin-8(IL-8)in tumor necrosis factor-alpha(TNF-a)-induced HT-29 cells,and had good self-aggregation ability and hydrophobicity.In conclusion,the two lactic acid bacteria strains MKLQ3807-13 and MKCH1884-43 have excellent probiotic properties,which can lay a foundation for the development of micro-ecological preparations for police dogs.[Chinese Journal of Animal Nutrition,2023,35(12):8133-8147]关键词
昆明犬/乳酸菌/分离鉴定/益生特性Key words
Kunming dog/lactic acid bacteria/separation identification/probiotic characteristics分类
畜牧业引用本文复制引用
李吉琴,曹振辉,展丽梅,自绍平,梅会友,高彦航,徐虎,徐乐,鲍国樱,李杰..昆明犬源乳酸菌的分离、鉴定与益生特性研究[J].动物营养学报,2023,35(12):8133-8147,15.基金项目
云南省中青年学术和技术带头人后备人才(202305AC160040) （202305AC160040）
