亚热带农业研究2023，Vol.19Issue(4)：247-253,7.DOI:10.13321/j.cnki.subtrop.agric.res.2023.04.005
5个甘蔗新品系叶绿素荧光参数的比较
Comparison on chlorophyll fluorescence parameters of 5 new sugarcane lines
摘要
Abstract
[Purpose]The photosynthetic physiological characteristics of 5 new sugarcane lines were studied to provide theoretical basis for the screening and cultivation of sugarcane germplasm with high light efficiency.[Method]Five sugarcane lines(Yunzhe 02588,Yunzhe 14658,Yunzhe 14599,Yunzhe 17613 and Yunzhe 17637)were determined on yield per unit and sucrose yield,as well as chlorophyll content(SPAD value)and chlorophyll fluorescence parameters of leaves by handheld portable SPAD-502 chloro-phyll meter and PAM-2500 portable fluorescence meter,based on which correlation analysis,principal component analysis,mem-bership function method and grey relational degree analysis were performed to comprehensively evaluate the 5 lines.[Result]There were significant or extremely significant differences in SPAD value and chlorophyll fluorescence parameters among the 5 lines.Corre-lation analysis showed that the initial fluorescence(F0)was significantly positively correlated with the maximum fluorescence(Fm),and the potential photochemical efficiency(Fv/F0)was significantly positively correlated with the maximum photochemical efficiency(Fv/Fm).The light capture efficiency of PSⅡ(ΦPSⅡ)was significantly positively correlated with photosynthetic quan-tum yield Y(Ⅱ)and photochemical quenching coefficient(q P),but negatively correlated with non-photochemical quenching coeffi-cient(q N).Principal component analysis showed that the cumulative contribution rate of the first 3 principal components reached 91.078%,which represented the most information of the total data.Grey relational degree analysis showed that Fm,F0 and Y(Ⅱ)had the highest correlations with yield per unit,and F0,q N and Y(Ⅱ)had the highest correlations with the theoretical sugar yield in November,while ΦPSⅡ and SPAD value had the lowest correlation with them.[Conclusion]Among the 5 sugarcane lines,Yunzhe 17637 and Yunzhe 14599 had higher comprehensive evaluation scores on photosynthetic physiological characteristics,and thus can be used as high-quality parent or germplasm with high light efficiency.关键词
甘蔗品系/叶绿素荧光参数/隶属函数法/主成分分析Key words
sugarcane line/chlorophyll fluorescence parameters/membership function method/principal components analysis分类
农业科技引用本文复制引用
俞华先,安汝东,刘家勇,董立华,桃联安,经艳芬,刘洪博..5个甘蔗新品系叶绿素荧光参数的比较[J].亚热带农业研究,2023,19(4):247-253,7.基金项目
云南省农业联合专项重点项目"糖分性状相关基因在甘蔗蔗糖分差异种间的功能解析"(202101BD070001-025) （202101BD070001-025）
国家重点研发计划项目子课题"高糖高产甘蔗新品种选育"(2022YFD2301100) （2022YFD2301100）
云南种子种业联合实验室项目(202205AR070001) （202205AR070001）
云南省农业科学院甘蔗种质创新及新品种选育省创新团队项目(2019HC013). （2019HC013）
