亚热带农业研究2023，Vol.19Issue(4)：268-272,5.DOI:10.13321/j.cnki.subtrop.agric.res.2023.04.008
福建七叶一枝花种质资源遗传多样性的ISSR分析
Genetic diversity of Paris polyphylla Smith var.chinensis germplasm resources from Fujian based on ISSR
摘要
Abstract
[Purpose]The study aimed to analyze the genetic diversity and phylogenetic relationship of Paris polyphylla Smith var.chinensis germplasm resources from Fujian Province,in order to quickly identify germplasm resources within and outside Fujian Province.[Method]Based on the result of ISSR marker amplification,the average Shannon diversity index and genetic similarity coefficient of P.polyphylla from Fujian Province were calculated by POPGENE 32 software and NTSYS,and phylogenetic relation-ship map was constructed using UPGMA clustering.[Result]Eight primers with stable,clear and highly polymorphic amplification bands were screened from 100 primers for ISSR-PCR amplification,and resulted in a total of 66 DNA bands,including 62 polymor-phic bands,with a polymorphism rate of 93.94%.The averages of Shannon diversity index,detected allele number,effective allele number,and Nei's gene diversity index for 27 germplasm resources were 0.377 9,1.939 4,1.372 0 and 0.237 7,respectively.The variation range of genetic similarity coefficients among 27 germplasm resources was from 0.63 to 0.88.UPGMA clustering showed that P.polyphylla populations from Fujian Province can be separated from those outside Fujian Province at a similarity coefficient of 0.63.When the genetic similarity coefficient was 0.69,27 germplasm can be divided into 4 major groups,in which group 1 was made up by 4 samples from Nanping City and 12 samples from Sanming City,group 2 was consisted of 7 samples from Longyan City,group 3 contained 3 samples from Jiangxi Province,Hunan Province and Hubei Province,and 1 sample from Sichuan Province clus-tered as group 4.[Conclusion]ISSR analysis revealed that P.polyphylla from Fujian Province had high genetic diversity,and the genetic relationship was related to geographical environment,which provided a certain reference basis for subsequent variety selec-tion,resource identification,and real estate region selection.关键词
七叶一枝花/ISSR分析/遗传多样性/UPGMA法/福建Key words
Paris polyphylla Smith var.chinensis/ISSR analysis/genetic diversity/UPGMA method/Fujian分类
农业科技引用本文复制引用
罗晓锋,周先治,周建金..福建七叶一枝花种质资源遗传多样性的ISSR分析[J].亚热带农业研究,2023,19(4):268-272,5.基金项目
福建省星火项目"华重楼棚架式集约化高效栽培技术示范"(2020S0020) （2020S0020）
"5511"协同创新工程项目"蔬菜、花卉优异种质资源创制与育种技术创新(药用植物)"(XTCXGC2021003). （药用植物）
