亚热带农业研究2023，Vol.19Issue(4)：273-281,9.DOI:10.13321/j.cnki.subtrop.agric.res.2023.04.009
微生物菌剂组合施用对艾草生长的影响
Effect of compound microbial agents on growth of Artemisia argyi
摘要
Abstract
[Purpose]The effect of compound microbial agents on the growth and physiological and biochemical indexes of Artemisia argyi were investigated to provide reference for its high-quality production.[Method]Trivariate quadratic regression orthogonal ro-tational combing design was adopted(1/2 implementation)to compare the effect of different combinations of Enterobacter aerogenes,Bacillus amyloliquefaciens and Acinetobacter calcoaceticus(T1-T16 treatment)on plant height,chlorophyll SPAD value,antioxidant enzyme activity,malonaldehyde(MDA)content,soluble sugar content and dry weight of aboveground part of Artemisia argyi seed-lings.Mathematical model and significance test were established and performed to depict the effect of compound microbial agents on superoxide dismutase(SOD)and catalase(CAT)activities,and dry weight of Artemisia argyi,followed by single factor effect anal-ysis and interaction effect analysis.[Result](1)In terms of growth indexes,the dry mass of Artemisia argyi was the biggest under T6 treatment,and the plant height was the highest under T8 treatment,both significantly higher than that of CK1 and CK2.(2)In terms of physiological and biochemical indexes,T6,T1 and T9 treatments were contributed to the highest SOD,CAT and peroxidase(POD)activity,respectively,which were also significantly higher than those of CK1 and CK2.Most combined treatments,except for T2,T4,T7 and T9 treatments,were resulted in significantly higher MDA contents of Artemisia argyi than those of CK1 and CK2.The contents of soluble sugar were less affected by compound microbial agents,presenting subtle variation trend among different treat-ments.Most combined treatments,except for T1,T4,T6 and T9 treatments,gave rise to significantly greater chlorophyll SPAD val-ues than CK1 and CK2.(3)Single factor effect analysis showed that the SOD activity of Artemisia argyi declined first and then in-creased with the application dosage of E.aerogenes,and gradually increased with the application dosage of Acinetobacter calcoaceticus.The CAT activity and dry mass of Artemisia argyi decreased first and then increased with the application dosages of E.aerogenes and B.amyloliquefaciens,and the CAT activity gradually increased with the application dosage of Acinetobacter calcoaceticus.Interaction effect analysis revealed that the interaction of E.aerogenes and B.amylolyticus was the most beneficial to the dry mass of Artemisia ar-gyi.[Conclusion]All combinations of the 3 microbial agents promoted the growth of Artemisia argyi,among which T6 treatment(80 mL E.aerogenes,40 mL B.amylolyticus and 40 mL Acinetobacter calcoaceticus)exerted the best effect and was recommended for wider application.关键词
微生物菌剂/艾草/生长指标/生理生化指标Key words
microbial agent/Artemisia argyi/growth index/physiological and biochemical index分类
农业科技引用本文复制引用
陈维峰,王思凡,马林,袁宗胜,刘芳..微生物菌剂组合施用对艾草生长的影响[J].亚热带农业研究,2023,19(4):273-281,9.基金项目
福建省科技计划项目"林下中药材生态栽培关键技术研究与应用"(2021I0043). （2021I0043）
