深部脑磁刺激改善大鼠卒中后抑郁样行为OACSTPCD

Objective:To investigate therapeutic effects of deep brain magnetic stimulation(DMS)on depressive-like behavior in a post-stroke depression(PSD)rat model and its potential mechanisms.Methods:Total 42 adult male SD rats were screened using sucrose preference test and open field test.They were randomly divided into sham surgery group(Sham group,n=6),stroke group(Stroke group,n=12),PSD group(n=12),and(PSD+DMS)group(n=12).Cerebral ischemia models were established induced by bilateral common carotid artery occlusion and reperfusion in groups stroke,PSD and(PSD+DMS).The common carotid artery of sham group was only separated without ligation.Groups PSD and(PSD+DMS)accepted chronic mild stress for 3 weeks,while(PSD+DMS)group rats accepted 40 Hz deep brain magnetic stimulation for 40 minutes per day for 2 weeks.The open field test was used to evaluate locomotor activity and anxiety-like behavior,while the sucrose preference test assessed anhedonia-like behavior.Immunofluorescence staining was performed to measure the expression level of Iba-1,a marker of glial cell activation,in the frontal lobe.Protein immunoblotting technique was used to detect the expression of CD 11b,a marker of glial cell activation,as well as inflammatory cytokines IL-1 β and TNF-α in the frontal lobe.Results:Treatment with DMS significantly improved depressive-like behavior in the(PSD+DMS)group compared to the PSD group.Glial cell activation and protein expression of inflammatory cytokines IL-1 β and TNF-α were increased in the frontal lobe of the stroke group.In the PSD group,further increase in glial cell activation and protein expression of IL-1 β and TNF-α was observed.However,in the(PSD+DMS)group,glial cell activation was reduced,and protein expression of IL-1 β and TNF-α was decreased.Conclusion:DMS treatment effectively improves depressive-like behavior in PSD rats.Inhibition of glial cell activation and expression of inflammatory cytokines in the frontal cortex may be potential mechanisms underlying its antidepressant effects.