华北农学报2023，Vol.38Issue(z1)：26-33,8.DOI:10.7668/hbnxb.20194138
陆地棉种质资源遗传多样性分析及初级核心种质构建
Genetic Diversity Analysis and Primary Core Germplasm Development of Upland Cotton Germplasm Resources
摘要
Abstract
The aim of this study was to evaluate the genetic diversity of upland cotton germplasm resources and screen representative germplasm,so as to provide theoretical basis for scientific evaluation and efficient utilization of upland cotton germplasm resources.Based on the SNP markers of 367 upland cotton resources and the phenotypic data of 353 resources,genetic diversities were analyzed,phylogenetic tree and primary core germplasm were con-structed,and effects of primary core germplasm construction were evaluated.The results showed that the polymor-phism information content(PIC)of the original upland cotton population was 0.24,and the genetic diversity index of each phenotypic trait was close to 2.00,which were similar to the results of previous studies.The upland cotton germplasm could be divided into three groups based on SNP markers,and 73 genotypic primary core resources were constructed.The Nei's genetic diversity index,PIC and other genetic diversity indexes of primary core germplasm were higher than those of original germplasm.Based on the phenotypic data,the upland cotton germplasm could be divided into three groups,and the mean value of each trait showed the trend that the groupⅠwas the smallest,the group Ⅱ was in the middle,and the groupⅢwas the largest.Seventy phenotypic primary core resources were con-structed.Compared with the original germplasm,the mean values of all traits were not significantly different,the range and genetic diversity index were similar to that of the original germplasm,and the coefficient of variation was higher than that of the original germplasm.Fifteen primary core resources constructed based on SNP markers over-lapped with those constructed based on phenotypic data.Most of these resources were genotypic group Ⅱand phe-notypic group Ⅱ.These results showed that the genotypic genetic diversity of upland cotton germplasm resources was low,but the phenotypic variation was rich and the phenotypic genetic diversity was high.The genotypic and phenotypic primary core germplasm constructed in this study had a good representation of genetic diversity and could be used as a typical germplasm for preservation and utilization.关键词
陆地棉/遗传多样性性/核心种质/SNP标记/表型性状Key words
Upland cotton/Genetic diversity/Core germplasm/SNP marker/Phenotypic traits分类
农业科学引用本文复制引用
张曦,刘祎,钱玉源,王广恩,王寒菊,焦秀芬,解辉,崔淑芳,李俊兰..陆地棉种质资源遗传多样性分析及初级核心种质构建[J].华北农学报,2023,38(z1):26-33,8.基金项目
河北省农林科学院科技创新专项(2022KJCXZX-MHS-4) （2022KJCXZX-MHS-4）
河北省现代农业产业技术体系棉花产业创新团队建设(HBCT2023070204) （HBCT2023070204）
