华北农学报2023，Vol.38Issue(z1)：39-48,10.DOI:10.7668/hbnxb.20194229
基于ISSR及SSR标记的苜蓿遗传多样性分析
Genetic Diversity Analysis of Alfalfa Based on ISSR and SSR Markers
摘要
Abstract
To explore the genetic relationship of alfalfa germplasm resources and provide theoretical basis for the identification of alfalfa varieties and the breeding of new varieties.Genetic diversity of 30 alfalfa materials was analyzed by ISSR and SSR markers.A total of 125 bands and 117 polymorphic bands were amplified by 12 ISSR la-beled primers.The polymorphic band ratio(PPB)was 93.01%.The average effective allele number(Ne),Nei's gene diversity index(H)and Shannon diversity index(I)were 1.465 9,0.281 3 and 0.431 4,respectively.A total of 152 bands and 144 polymorphic bands were amplified by 12 SSR markers,and the polymorphism band ratio(PPB)was 94.05%.The average effective allele number(Ne),Nei's gene diversity index(H)and Shannon diversi-ty index(I)were 1.542 7,0.313 9 and 0.470 1,respectively.The genetic similarity coefficient and cluster analysis of the two markers showed that Zxy2010p-7900 and Zxy2010p-7740 could be divided into a separate category and were far related to other materials.Qingshui alfalfa,Longdong alfalfa and Gannong No.4 mixed alfalfa can be divid-ed into one group,the genetic similarity coefficient between the varieties is large,and the relationship is close.The phylogenetic relationship of the tested alfalfa germplasm was objectively determined by the two labeling methods.关键词
苜蓿/ISSR/SSR/遗传多样性/亲缘关系Key words
Alfalfa/ISSR/SSR/Genetic diversity/Genetic relationship分类
农业科技引用本文复制引用
马彪,南丽丽,姚宇恒,夏静,汪堃,陈洁,何海鹏..基于ISSR及SSR标记的苜蓿遗传多样性分析[J].华北农学报,2023,38(z1):39-48,10.基金项目
国家自然科学基金(32160327) （32160327）
甘肃省科技计划(22YF7NA112) （22YF7NA112）
财政部和农业农村部:国家现代农业产业技术体系(CARS-34) （CARS-34）
