华北农学报2023，Vol.38Issue(z1)：49-57,9.DOI:10.7668/hbnxb.20194251
桃叶片低温响应转录组分析
Transcriptome Analysis of Low Temperature Response in Peach Leaves
摘要
Abstract
Cold resistance is an important target trait for peach breeding and is regulated by multiple genes.Transcriptome sequencing is used to sequence transcripts under specific physiological conditions to explore the mo-lecular mechanism of low temperature response of peach leaves.Leaves of Xiongyuejutao under 4℃low temperature(LT)treatment and normal leaves(RT)were analyzed by transcriptome sequencing to explore the molecular regula-tion mechanism of cold resistance.A total of 72 up-regulated differentially expressed genes and 3 down-regulated genes were obtained by DESeq2.KEGG analysis was used to analyze the functions of differentially expressed genes in MAPK signaling pathway and plant-pathogen interaction pathway,and protein interaction was predicted.The re-sults showed that leaves under low temperature treatment showed physiological states of curled,yellow,and water loss.The differentially expressed genes were mainly enriched in plant-pathogen interaction,MAPK signaling path-way,and secondary biomass accumulation.MYC2,SPCH and Pti4-6 transcription factors may play an important role in the regulation of cold-induced injury in response to cold stress.MYC2 and CBF proteins were predicted to in-teract with each other and interacted with multiple proteins.The results showed that multiple metabolic pathways re-sponded to low temperature stress in peach leaves,and related transcription factors that may play a key role in the regulation of cold response pathways were predicted,which provided research direction and basis for the exploration of molecular mechanism of cold response regulation in peach.关键词
桃/叶片/低温/转录组分析Key words
Peach/Leaf/Low temperature/Transcriptome analysis分类
农业科技引用本文复制引用
时梦,冀晓昊,杨兴旺,王莹莹,王海波,王孝娣..桃叶片低温响应转录组分析[J].华北农学报,2023,38(z1):49-57,9.基金项目
中央级公益性科研院所基本科研业务费专项(Y2021YJ23) （Y2021YJ23）
中国农业科学院创新工程项目(CAAS-ASTIP-2015-RIP-06) （CAAS-ASTIP-2015-RIP-06）
国家重点研发计划(2020YFD1000203) （2020YFD1000203）
