华北农学报2023，Vol.38Issue(z1)：67-77,11.DOI:10.7668/hbnxb.20193901
低温胁迫对普通越冬栽培稻幼苗耐寒生理生化指标的影响
Effect of Low Temperature Stress on Physiological and Biochemical Indices of Cold Tolerance in Common Overwintering Cultivated Rice Seedling
摘要
Abstract
The physiological and biochemical indices of cold tolerance in common overwintering cultivated rice seedling were studied for providing theoretical basis for the innovation and utilization of overwintering cultivated rice germplasm.A total of 20 overwintering cultivated rice germplasms were selected as experimental materials,altogeth-er five physiological and biochemical indices such as chlorophyll,malondialdehyde and SOD activity in rice seedling were measured and analyzed under both normal temperature(25℃)and 4 days of low temperature(4℃)stress.The five cold tolerance indices under normal temperature condition displayed extremely significant phenotypic differ-ences among all tested materials from seven different provinces in China.The cold tolerance index such as chloro-phyll and soluble sugar content apparently declined after chilling stress,but exhibited an inconsistent rate of decline among all experimental materials,ranged from-36.46%to-90.31%,-70.54%to-94.03%,respectively.While the cold tolerance indices such as malondialdehyde,proline and SOD activity in all tested rice seedlings dis-played disparate response to cold chilling and exhibited an inconsistent rate of rising or falling.Among all tested materials,the malondialdehyde content in three tested materials such as Huangnuo 2,Wanjingnuo M99037 and Changnongjing 3 exhibited significantly decline after chilling stress,the remaining seventeen tested materials exhibi-ted a significant rise.The proline content in eight tested materials such as Tiejingdao 4,Zhendao 7 and Jindao 1187 exhibited a significantly rising after being chilling stress,but the remaining twelve tested materials exhibited a signif-icant decline malondialdehyde content value.The SOD activity in Ewandao 15 and Jinjingzasi exhibited an incon-spicuous decline after chilling stress,while the SOD activity in the remaining eighteen tested materials exhibiteda significant rise.Only 2 pairs of extremely significant PCC values among the five cold tolerance indices were calcu-lated under the normal temperature environment,while 8 pairs of extremely significant PCC values were calculated after chilling stress.Apparently,the number,strength and direction of correlations among the 5 cold tolerance indi-ces were easily varied after chilling stress.On the whole,the cold tolerance indices in rice seedling under both nor-mal temperature and low temperature exhibited significant difference owing to the different genetic background of o-verwintering cultivated rice.关键词
越冬栽培稻/低温胁迫/生理生化指标Key words
Overwintering cultivated rice/Chilling stress/Physiological and biochemical index分类
农业科技引用本文复制引用
严雨欣,龚俊义,彭廷燊,秦小健,南文斌,张汉马,梁永书..低温胁迫对普通越冬栽培稻幼苗耐寒生理生化指标的影响[J].华北农学报,2023,38(z1):67-77,11.基金项目
西南作物基因资源发掘与利用国家重点实验室"生物育种"揭榜挂帅重点项目(SKL-KF202226) （SKL-KF202226）
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0007) （cstc2021jcyj-msxmX0007）
