华北农学报2023，Vol.38Issue(z1)：188-193,6.DOI:10.7668/hbnxb.20193918
外源γ-氨基丁酸对高温胁迫下番茄幼苗生长、抗氧化酶活性及光合特性的影响
Effects of Exogenous GABA on Growth,Leaf Antioxidant Enzyme Activity and Photosynthetic of Tomato Seedlings under High Temperature Stress
摘要
Abstract
In order to explore the effects of different concentration of exogenous GABA on tomato seedlings dry matter accumulation,root development,antioxidant enzyme activities and photosynthetic characteristics under high temperature stress,the tomato variety Fenteli was selected as the test material,and five treatments,including spra-ying distilled water(CK),1 mmol/L GABA(T1),5 mmol/L GABA(T2),10 mmol/L GABA(T3)and 20 mmol/L GABA(T4),were set up in this study.Spraying once every two days for two consecutive times.After spraying,the seedlings were exposed to high temperature stress at 40℃/35℃(day/night),14 h(light)/10 h(dark)with light intensity of 250 μmol/(m2·s)for 7 days.The results showed that compared with CK,spraying GABA with the con-centration of 10 mmol/L or less(T1,T2 and T3)significantly promoted the growth of tomato seedlings,and the plant height,stem thickness,aboveground dry weight and seedling index increased by 4.96%—6.92%,7.65%—19.26%,29.41%—52.94%,20.00%—26.67%,respectively.Noteworthy,compared with CK,T2 treatment sig-nificantly increased the total root length,total surface area,total volume and tip numbers of root system by 23.04%,13.95%,11.76%and 18.30%,respectively.Moreover,compared with CK,T2 treatment not only increased the leaves activities of SOD,POD and CAT in(increasing by 22.58%,51.73%and 148.80%respectively),but also decreased the relative conductivity and malondialdehyde content(decreasing by 12.84%and 21.89%),and thus significantly increasing the photosynthetic rate,stomatal conductance and transpiration rate of leaves(increasing by 81.38%,36.19%and 41.26%respectively)by ensuring the integrity of the cell membrane structure and function of leaves.However,there were no significance differences for the dry matter accumulation,photosynthetic character-istics and relative conductivity of tomato seedlings under T4 and CK treatments,and even the former inhibited the root development.In a word,our study demonstrated that foliar spraying with an appropriate GABA concentration could alleviate the inhibition of photosynthesis under high temperature stress through protecting the integrity of leaf cell membrane by improving capacity of leaf osmotic adjustment and antioxidant enzyme activities.Additionally,spraying GABA could also improve the heat tolerance of tomato seedlings by promoting dry matter accumulation and root development.The effect of GABA with 5 mmol/L was the best in this study.关键词
番茄幼苗/γ-氨基丁酸/高温胁迫/抗氧化酶活性/光合特性Key words
Tomato seedlings/GABA/High temperature stress/Antioxidant enzyme activity/Photosynthetic characteristics分类
农业科技引用本文复制引用
史建硕,潘丽佳,蒋龙刚,贾舟,任燕利,侯升林,王丽英..外源γ-氨基丁酸对高温胁迫下番茄幼苗生长、抗氧化酶活性及光合特性的影响[J].华北农学报,2023,38(z1):188-193,6.基金项目
河北省农林科学院基本科研业务费项目(2021130101) （2021130101）
河北省省级科技计划项目(21326904D) （21326904D）
省现代农业产业技术体系露地/设施蔬菜创新团队水肥资源高效利用岗(HBCT2021200201) （HBCT2021200201）
河北省现代农业产业技术体系露地/设施蔬菜创新团队智能化管理及综合提升岗(HBCT2021200202) （HBCT2021200202）
