华北农学报2023，Vol.38Issue(z1)：203-210,8.DOI:10.7668/hbnxb.20193808
铺设反光膜促进设施葡萄着色的机理初探
Preliminary Study on the Mechanism of Laying Reflective Film to Promote the Coloring of Protected Grape
摘要
Abstract
To find out the effect of laying reflective film on fruit quality in facilities and the mechanism of pro-moting coloring,the changes of the appearance and anthocyanin content of Jingyan grape fruits were monitored with the film laid between rows,then transcriptome sequencing and differential expression gene analysis of the peels with different coloring were carried out using high-throughput sequencing and Illumina Novaseq 6000 sequencing plat-form.Finally,41 151 986-46 673 908 Clean reads were obtained from 12 samples,with Q30 was over 94%and Q20 was over 98%.Besides,the mapping ratio between Clean reads and grape reference genome was 92.69%—94.22%.The analysis results of differentially expressed genes(DEGS)showed that there were 3 768 DEGs in CK 0 d—CK 7 d,with 1 488 up-regulated and 2 280 down-regulated.And 5 129 in RF 0 d—RF 7 d after film mulc-hing,with 2 048 up-regulated and 3 081 down-regulated.It showed that film mulching can increase the differential expression of genes.Compared with CK,934 DEGs were obtained after 7 days mulching,of which 441 genes were up-regulated and 493 were down-regulated.KEGG enrichment analysis showed that,a total of 179 DEGs were anno-tated on 64 signal pathways,which were mainly enriched in five metabolic pathways:metabolism,cellular process,environmental information processing,genetic information processing and organismal systems.Particularly,many genes vere differentially expressed in benzene propane biosynthesis,endoplasmic reticulum protein processing,plant pathogen interaction and plant hormone signaling pathway.Results showed some transcripts of PAL,F3'H and 3GT were up-expressed,and some auxin-related protein genes were down-regulated.For transcription factors,WRKY gene was mainly up-regulated,while bHLH,C2H2,NAC,MIKC-MADS,Dof were down-regulated.In conclusion,laying reflective film may improve the activity of phenylalanine ammonia-lyase(PAL)and the expression of related genes(F3'H,3GT),thus promoting anthocyanin synthesis.Meanwhile,transcriptional factors WRKY,bHLH,C2H2,MYB,NAC,MIKC-MADS and Dof may also play an important role in regulation.关键词
葡萄/反光膜/着色/转录组/差异表达基因Key words
Grape/Reflective film/Colour/Transcriptome/DEGs分类
农业科技引用本文复制引用
牛早柱,赵艳卓,陈展,宣立锋,牛帅科,李亚囡,杨丽丽..铺设反光膜促进设施葡萄着色的机理初探[J].华北农学报,2023,38(z1):203-210,8.基金项目
国家现代葡萄产业技术体系(CARS-29-3) （CARS-29-3）
河北省现代农业产业技术体系葡萄创新团队(HBCT2021230202) （HBCT2021230202）
热杂果现代种业科技创新团队(21326310D) （21326310D）
河北省农林科学院创新工程项目(2022KJCXZX-SGS-7) （2022KJCXZX-SGS-7）
