华北农学报2023，Vol.38Issue(z1)：245-252,8.DOI:10.7668/hbnxb.20193807
基于代谢组学的板栗叶片功能性物质筛选与分析
Screening and Analysis of Functional Substances in Chinese Chestnut Leaves Based on Metabolomics
摘要
Abstract
To provide a theoretical basis for the resource utilization of Chinese chestnut,the mature leaves of four chestnut cultivars were tested using ultra high performance liquid chromatography-tandem mass spectrometric,and func-tional secondary metabolites were screened by consulting relevant pharmacopoeias and searching the Chemical Book,Chemsrc,pharmacopeias and Web of Science database.The contents of 18 secondary metabolites we focused on in differ-ent cultivars were compared and analyzed.The study showed that a total of 673 kinds of secondary metabolites were de-tected in chestnut leaves,including flavonoids,phenolic acids,alkaloids,tannins,terpenoids,lignin and coumarin.Princi-pal component analysis and cluster analysis showed that the difference of secondary metabolomics profile between Yans-hanzaofeng and Yankui was little.The major secondary metabolites contributing to the classification were phloretin,didy-min,salirepin,arbutin,cinnamic acid,gallocatechin,procyanidin B1 and so on.The differential metabolites could also be used as biomarkers to distinguish different chestnut varieties.A total of 202 functional secondary metabolites were select-ed,including 51 phenolic acids,24 alkaloids,83 flavonoids,21 terpenoids,14 lignins and coumarins,and 9 tannins.The contents of active substances were high in leaves such as protocatechuic acid,maslinic acid,ursolic acid,ellagic acid,chlorogenic acid,avicularin,kaempferol-7-O-glucoside,quercetin,spiraeoside,astragalin,betanin,tiliroside,ditymin and baimaside,which provided reference for the resource utilization of Chinese chestnut leaves.关键词
板栗/叶片/次生代谢物/功能性物质Key words
Chinese chestnut/Leaf/Secondary metabolite/Functional substance分类
农业科技引用本文复制引用
张馨方,张树航,李颖,郭燕,王广鹏,王英杰..基于代谢组学的板栗叶片功能性物质筛选与分析[J].华北农学报,2023,38(z1):245-252,8.基金项目
河北省农林科学院农业科技创新专项(200KJCXZK-RW-5) （200KJCXZK-RW-5）
河北省科技计划项目(21326304D-3) （21326304D-3）
河北省自然科学基金(C2020301053) （C2020301053）
