华北农学报2023，Vol.38Issue(z1)：400-407,8.DOI:10.7668/hbnxb.20193874
猪CRYBB2基因启动子的克隆、活性及转录调控元件分析
Cloning,Transcriptional Activity and Regulatory Elements Analysis of Porcine CRYBB2 Gene Promoter
摘要
Abstract
The purpose of this study was to preliminarily analyze the promoter activity and transcription regula-tory elements of porcine CRYBB2.Using bioinformatics analysis,PCR amplification,gene cloning,cell transfection,double luciferase activity analysis and other methods,we obtained the sequence characteristics of CRYBB2 promoter region,constructed double luciferase reporter gene vectors of CRYBB2 promoter region with different fragment lengths,analyzed its luciferase activity,and then determined the core promoter region and key regulatory region of CRYBB2.Finally,the transcription factors and their binding sites in key regulatory regions were predicted.The results showed that the candidate promoter region of CRYBB2 might contain four core promoters and one CpG island.-52—-3 bp might be the core promoter region of CRYBB2 gene,and-505—-19 bp might be the key regulatory region of CRYBB2 gene promoter,which played a positive regulatory role,while-2 060—-505 bp didn't have any regulatory elements that affected the activity of CRYBB2 gene promoter.The key regulatory region of CRYBB2 promoter contained multiple potential transcription factor binding sites,such as TBP,NFIA,FOXP1,NKX2-8,KLF4,Tcf3,Crx,SNAI3,Rfx1 and CREB1.This study laid a foundation for further study on the expression mechanism of porcine CRYBB2.关键词
猪/CRYBB2/启动子/转录活性/转录因子Key words
Pig/CRYBB2/Promoter/Transcriptional activity/Transcription factor分类
农业科技引用本文复制引用
龙熙,袁晓,陈力,吴平先,张亮,潘红梅,郭宗义,柴捷..猪CRYBB2基因启动子的克隆、活性及转录调控元件分析[J].华北农学报,2023,38(z1):400-407,8.基金项目
重庆市科研院所绩效激励引导专项(21530) （21530）
国家重点研发计划项目(2021YFD1301105) （2021YFD1301105）
