辣椒交替氧化酶全基因家族的鉴定及表达分析OA北大核心CSTPCD

In order to explore the role of the pepper alternative oxidase(AOX)gene in plant growth and development and resistance to stress,12 pepper AOX genes were screened and identified from the whole genome of pepper by bioinformatics methods such as blast alignment and binding domain analysis.They were named as CaAOX1c11,CaAOX1a19,CaAOX1b11,CaAOX2d11,CaAOX2a16,CaAOX2a11,CaAOX2c13,CaAOX2a18,CaAOX2b13,CaAOX2a13,CaAOX2a22 and CaAOX2b19.The bioinformatics analysis was carried out and the expression patterns of CaAOXs in different tissues,stress and hormone response of pepper were explored.The results showed that 12 CaAOXs could be divided into two subfamilies,AOX1 and AOX2.The number of amino acids encoded by CaAOXs ranged from 146 to 513,and the isoelectric point ranged from 4.41 to 8.80.The CaAOXs were unevenly distributed on different chromosomes and not secretory proteins,most of which were located in mitochondria,and a few were located in chloroplasts and nuclei.They have a relatively stable conserved domain and tertiary structure,and the AOX functional domain was very conserved at the C-terminus.Quantitative real-time PCR(qRT-PCR)results showed that the expression of CaAOXs was up-regulated in flowers and leaves,and the expression level was the highest in flowers.Most of the CaAOXs were up-regulated under GA and ABA treatments,and the response to ABA was stronger.The expression of CaAOX2b19,CaAOX1c11,and CaAOX2a22 was up-regulated under low and high temperature stress,and CaAOX1c11 strongly responded to high temperatures.The above results indicated that CaAOXs may be involved in the growth and development of pepper,response to stress,and hormone.The results of this study laid a foundation for further study on the role of CaAOXs in improving the stress resistance of pepper.