致病疫霉菌效应蛋白Pi05440毒性功能验证及寄主候选靶标筛选OA北大核心CSTPCD

For successful colonization,Phytophthora infestans secretes a variety of effector proteins into plant cells to facilitate infection.To study the function of the effector protein Pi05440 of P.infestans,this study focused on the Pi05440 characteristics,virulence function and its host candidate targets screening.The conserved domain and signal peptide of Pi05440 were analyzed by using a variety of databases.The expression vector pRI101-GFP-Pi05440 was constructed for subcellular localization analysis.To further explore host candidate proteins interacting with Pi05440,yeast cDNA library were screened using yeast two-hybrid.Sequence analysis showed that gene Pi05440 was 969 bp in length and encoded 322 amino acids.Conservative structure analysis suggested that Pi05440 contained three typical KAZAL functional domains.Transient expression indicated that Pi05440 was located in the plasma membrane and intercellular space,and significantly promoted P.infestans colonization in Nicotiana benthamiana.In addition,three candidate target proteins,peroxidase 12,gibberellinase and MYB-like A protein were initially identified by yeast two-hybrid screening.This work will provide important clues for further investigation on how P.infestans effector Pi05440 and the associated target proteins modulate host immunity.