特色香料作物油樟叶内生真菌多样性及其挥发性成分分析OA北大核心CSTPCD

In order to explore the endophytes fungi of Cinnamomum longepaniculatum,a special spice crop,as subjects,the essential oil of leaves of five Cinnamomum longepaniculatum lines in the four seasons was extracted and cultivatable endophytes were isolated from leaves and purified.Then,the endophyte abundance was analyzed based on the diversity index by using ITS sequencing,and the volatile components of fermentation broth were identified using GC/MS.The results showed that the oil yield of different Cinnamomum longepaniculatum varieties in different seasons were varied,and the oil yield of No.16 in spring was relatively high.A total of 43 cultivatable strains of endophytes were acquired by isolation and purification,and could be divided into two phyla,four classes,nine orders,11 families,and 13 genera;the dominant genus was Phyllosticta.Analysis of diversity and uniformity revealed that the endophyte population diversity and uniformity from variety No.16 in spring were higher than those from other strains.Additionally,69 volatile components were identified in the endophyte fermentation broth by the GC/MS method,wherein X29,D72 and A39 can generate essential oil of Cinnamomum longepaniculatum leaves.The quantity,population and composition of cultivatable endophytes were dependent on the strain and the season,and the identified functional strains show the potential for developing of spice components.The results of this study provide theoretical basis and materials for the subsequent development and research of spice-producing substances.