检验医学与临床2024，Vol.21Issue(2)：213-216,220,5.DOI:10.3969/j.issn.1672-9455.2024.02.016
儿童重症肺炎支气管肺泡灌洗液的病原菌及药敏试验分析
Analysis of pathogens and drug sensitivity tests of bronchoalveolar lavage fluid in children with severe pneumonia
摘要
Abstract
Objective To investigate the pathogens and drug sensitivity of bronchoalveolar lavage fluid(BALF)in children with severe pneumonia.Methods A total of 100 children with severe pneumonia admitted to the hospital from January 2021 to April 2023 were selected as the research objects.Bronchoscopic alveolar lavage was performed on all children,and the pathogen and drug sensitivity test of BALF were analyzed.Results A total of 131 strains of pathogenic bacteria were cultured in BALF of 100 children,including 98 strains of Gram-negative bacteria(74.81％)and 33 strains of Gram-positive cocci(25.19％),of which Hae-mophilus influenzae(25.19％)and Streptococcus pneumoniae(21.37％)were relatively high.Branhamia ca-tarrhalis(15.27％),Klebsiella pneumoniae(11.45％)followed.The top four pathogens in BALF of children with severe pneumonia were mainly＜1 year old(97.37％)and 1-3 years old(96.88％),while＞3-7 years old(94.12％)and＞7-14 years old(92.31％)were relatively less.There was no significant difference in the distribution of Haemophilus influenzae,Branhamella catarrhalis and Klebsiella pneumoniae in BALF between different ages(P＞0.05).There was a significant difference in the distribution of Streptococcus pneumoniae in BALF among different age groups(P＜0.05).Twenty Haemophilus influenzae strains were sensitive to tetracycline,amoxicillin-clavulanic acid,cefotaxime and compound sulfamethoxazole,followed by chloram-phenicol,and highly resistant to ampicillin,cefaclor and cefuroxime.Fifteen Branhamia catarrhalis strains were more sensitive to cefotaxime,and most resistant to ampicillin.The 8 Klebsiella pneumoniae strains were sensi-tive to cefotaxime,and highly resistant to ampicillin,cefuroxime and chloramphenicol,followed by amoxicillin-clavulanic acid and tetracycline.The 20 strains of Streptococcus pneumoniae were sensitive to vancomycin and chloramphenicol,followed by penicillin G and telithromycin.The resistance to compound sulfamethoxazole was high,and the resistance to tetracycline,cefotaxime and clindamycin was second.Conclusion Haemophilus influenzae and Streptococcus pneumoniae are the most common pathogens causing severe Haemophilus influ-enzae and Streptococcus pneumoniae are the most common pathogens in BALF of children with severe pneu-monia,and the proportion of pathogens in children aged 0 to 3 years is higher than that in children aged＞3 to 14 years.Clinical drug use should be rational to prevent drug resistant strains.关键词
重症肺炎/儿童/支气管肺泡灌洗液/抗菌药物/药敏试验/革兰阴性菌/革兰阳性球菌Key words
severe pneumonia/children/bronchoalveolar lavage/antimicrobial agents/drug sensi-tivity test/Gram-negative bacteria/Gram-positive cocci分类
医药卫生引用本文复制引用
尤玉婷,曾丽娥,林春燕,陈琼华,林洁如,郑敬阳..儿童重症肺炎支气管肺泡灌洗液的病原菌及药敏试验分析[J].检验医学与临床,2024,21(2):213-216,220,5.基金项目
福建省泉州市医疗卫生领域指导性科技计划项目(2021N091S). （2021N091S）
