环境与职业医学2024，Vol.41Issue(1)：96-102,7.DOI:10.11836/JEOM23283
基于热解吸-气相色谱质谱法测定呼出气中27种挥发性有机物浓度
Determination of 27 volatile organic compounds in exhaled breath by thermal desorption-gas chromatography-mass spectrometry
摘要
Abstract
[Background]Volatile organic compounds(VOCs)in exhaled breath are closely associated with respiratory diseases and are linked to various metabolic reactions in the human body.A quanti-tative analytical method can provide technical support for studying VOCs related to various dis-eases. [Objective]To establish a thermal desorption-gas chromatography-mass spectrometry(TD-GC-MS)method for the determination of 27 VOCs in exhaled breath. [Methods]VOCs in exhaled breath were collected using a Bio-VOC sampler and enriched with Tenax TA thermal desorption tubes before TD-GC-MS analysis.Standards were collected using thermal desorption tubes and optimized for thermal desorption conditions as well as chromato-graphic and mass spectrometric conditions:The separation of the 27 VOCs was achieved by an optimized temperature program,the improvement of sensitivity by optimizing quantitative ions,and the increase of VOCs desorption efficiency by optimizing thermal desorption time and temperature.Limit of detection,limit of quan-tification,accuracy,precision,and stability of the proposed method were investigated by spiking with a blank gas bag,and exhaled breath samples from 20 healthy individuals were collected for an application study of the proposed method. [Results]The thermal desorption temperature was 280℃,and desorption time was 6 min.A VF-624ms chromatographic column was se-lected for the separation of target substances.The initial temperature of heating program was 35℃,maintained for 1 min,and then in-creased to 100℃at a heating rate of 3℃·min-1 for 1 min,followed by increasing to 210℃at a heating rate of 28℃·min-1 for 5 min.A quantitative analysis was conducted with a single ion monitoring(SIM)mode.Under these conditions,the 27 VOCs showed good linear relationships in their respective concentration ranges and the correlation coefficients were higher than 0.9990.The limits of detection of the method were in the range of 0.01-0.13 nmol·mol-1,the limits of quantification were in the range of 0.02-0.44 nmol·mol-1,and the spiked recoveries were in the range of 80.1%-120.5%,with intra-batch and inter-batch precision≤18.8%and 17.9%respectively.All sub-stances can be stored at room temperature(23-28 ℃)for 7 d and at 4 ℃ for 14 d.The proposed method was applied to exhaled breath samples from 20 subjects with detection rates≥80%(except for trans-2-pentene and decane)and a concentration range of 0.00-465.50 nmol·mol-1. [Conclusion]The established TD-GC-MS method for quantification of VOCs in exhaled breath is characterized by high sensitivity and good accuracy,and is suitable for quantitative determination of VOCs in exhaled breath,which can provide technical support for the study of exhaled breath VOCs.关键词
呼出气/挥发性有机物/热解吸/气相色谱-质谱Key words
exhaled breath/volatile organic compound/thermal desorption/gas chromatography-mass spectrometry分类
医药卫生引用本文复制引用
高汭,彭方达,何雅珍,冯洁,刘武宾,于丽佳,陈青松,丁春光..基于热解吸-气相色谱质谱法测定呼出气中27种挥发性有机物浓度[J].环境与职业医学,2024,41(1):96-102,7.基金项目
国家卫生健康委职业安全卫生研究中心自管项目(2019009) （2019009）
中央级公益性科研院所基本科研项目(2020-PT320-005) This study was funded. （2020-PT320-005）
