四川大学学报（自然科学版）2024，Vol.61Issue(1)：186-193,8.DOI:10.19907/j.0490-6756.2024.016002
孕激素相关子宫内膜蛋白在胃癌早期诊断、预后中的作用
The role of Progestagen-associated endometrial protein in early diagnosis and prognosis of gastric cancer
摘要
Abstract
To use a bioinformatics system to analyze the early diagnosis and prognosis prediction per-formance and immune infiltration correlation of Progestagen-associated endometrial protein(PAEP)in gastric cancer,this study constructed a predictive model ROC curve and deviation correction curve to test its performance accuracy,and further validate the analysis results through cell experiments.The re-sults of bioinformatics analysis showed that the expression of PAEP in gastric cancer tissues was higher than that in normal tissues,which was statistically significant in the early stages(T1,N0,M0)(P＜0.001),and the diagnostic accuracy of gastric cancer was higher(the area under ROC curve was 0.889).The prognosis of gastric cancer patients with high expression of PAEP was poor(P≤0.001).The later the TNM stage,the older the age,the higher the expression of PAEP,and the lower the sur-vival probability of gastric cancer patients,and the deviation correction curve was close to the ideal curve(45 ° line),indicating a good predictive result.The expression of PAEP was positively correlated with the infiltration level of neutrophils and macrophages in gastric cancer,and negatively correlated with B cells,central memory T cells and T cells.qRT-PCR and Western blot results showed that the transcrip-tional and protein expression levels of PAEP in HGC-27,BGC-823,MKN-45,SGC-7901 and AGS cells were significantly higher than those in GES-1 cells.The results indicate that PAEP protein can be used as a biomarker in the diagnosis and prognosis of gastric cancer,which is verified by experiments,and its mechanism is related to immunity.关键词
胃癌/孕激素相关子宫内膜蛋白/TCGA/生存分析/免疫浸润Key words
Gastric cancer/Progestagen-associated endometrial protein/TCGA/Survival analysis/Im-mune infiltration分类
医药卫生引用本文复制引用
张保根,杨澍,陈斌妮,陈文,郑梓莹,张燕..孕激素相关子宫内膜蛋白在胃癌早期诊断、预后中的作用[J].四川大学学报（自然科学版）,2024,61(1):186-193,8.基金项目
福建省中医药科研课题(2017FJZYLC502) （2017FJZYLC502）
