生命科学仪器2023，Vol.21Issue(5)：1-7,7.DOI:10.11967/2023211001
便携式实时荧光定量PCR仪数据处理方法研究
Research on Data Processing Methods for Portable Real time Fluorescence Quantitative PCR Instrument
摘要
Abstract
Real time fluorescence quantitative PCR is increasingly being used to quantify the expression level of nu-cleic acids.With the development and progress of the economy and society,individuals'self-protection awareness and personal health have become more important,which has led to higher demands for health.The demand for on-site nucleic acid analysis technology is becoming increasingly significant,and different instruments have different robust and suitable measurement and analysis methods.This study focuses on the data processing and analysis of a portable real-time fluorescence quantitative PCR instrument based on turntable fluorescence detection.By compa-ring and optimizing different peak fitting algorithms and amplification curve fitting algorithms,the detection per-formance of the optical system has been improved.The results show that the peak fitting algorithm for the system uses sine fitting to process the original data has a good effect.The amplification curve fitting algorithm uses 4-pa-rameter logistic fitting.The linear Goodness of fit of the amplification results of standard concentration gradient samples is R2＞0.990,and the CV value of the coefficient of variation is less than 5％.This improves the detection performance of the optical system,and provides some reference for the data processing methods of this type of de-tection model instrument.关键词
实时荧光定量PCR/数据处理/曲线拟合/扩增曲线Key words
Real time fluorescence quantitative PCR/Data processing/Curve fitting/Amplification curve分类
能源科技引用本文复制引用
侯红渠,邓玉林,樊云龙,吕雪飞,李晓琼..便携式实时荧光定量PCR仪数据处理方法研究[J].生命科学仪器,2023,21(5):1-7,7.基金项目
国防基础科研计划项目(JCKY2021602B009) （JCKY2021602B009）
