摘要
Abstract
This study investigated the effect of extract of damiana and black pepper(EDBP)on oligozoospermia in zebrafish model.The maximum tolerance concentration of EDBP was determined to be 500 μg/mL.Male zebrafish(10mpf)were randomly divided into model control group,positive drug group,EDBP-250 μg/mL group,EDBP-500 μg/mL group and normal control group.To each dosing group,EDBP was soaked for 8 days,and then cy-clophosphamide was added for 8 days at the same time.Growth and development parameters,sperm quality,plas-ma hormone levels and nucleic acid expression of CYP17α2,lhcgr and fshr were measured.The results showed that the body length,body weight,testis weight,sperm vitality and number,plasma testosterone(T),follicle stimula-ting hormone(FSH)and luteinizing hormone(LH)in the model control group were significantly reduced compared with the normal control group(p＜0.001).The expression levels of CYP17α2,lhcgr and fshr in seminal nest tis-sues were significantly reduced.The test results in EDBP groups were significantly higher than those in model con-trol group(p＜0.001),and the 500 μg/mL dose group was better.Therefore,EDBP can obviously improve oligo-spermia of zebrafish.关键词
达迷草叶/少精症/斑马鱼/环磷酰胺Key words
damiana/oligospermia/zebrafish/cyclophosphamide分类
医药卫生
