生命科学仪器2023，Vol.21Issue(5)：31-37,7.DOI:10.11967/2023211006
基于导数-恒基体同步荧光的纺织品中萘胺同分异构体快速定量检测
Rapid quantitative detection of naphthylamine isomers in textiles based on Derivative-Matrix Isopotential Synchronous Fluorescence
摘要
Abstract
2-naphthylamine(2-NA)is a prohibited carcinogenic aromatic amine in textiles.It is similar in struc-ture and properties to the isomer 1-naphthylamine(1-NA)which is non-prohibited,while traditional detection methods may cause false positive results when testing textile sample.The relevant standards require the use of multiple methods for re-examination and confirmation when a positive result is detected.And the current conven-tional re-examination methods are gas chromatography-mass spectrometry and high performance liquid chroma-tography,which have disadvantages such as complicated operation and time-wasting.It is of great importance to develop a simple and rapid method for the identification and quantification of carcinogenic aromatic amines and their isomers in textiles.Derivative matrix isopotential synchronous fluorescence spectroscopy has the characteristics of high sensitivity,good selectivity and elimination of background interference,and is widely used in the determination of substances in complex samples.In order to realize the rapid identification and quantification of 1-NA and 2-NA in textiles,a synchronous fluorescence analysis method combined with derivative technology was established.Establishing a proper scanning trajectory of matrix isopotential synchronous fluorescence spectroscopy(MISFS)can improve spectral resolution of isomers.When MISFS combined with first derivative technique,the background interference is eliminated and the sensitivity and selectivity are both improved.Meanwhile,time-wasting physical separation process and multi-scanning are avoided.The scanning time is about 2 min.According to the experi-ment results,the detection limits for 1-NA and 2-NA are 0.001 mg/L(corresponding to 0.1 mg/kg in textiles)and 0.006 mg/L(corresponding to 0.6 mg/kg in textiles),respectively.And the spiked recoveries of 1-NA and 2-NA are 89.1-122.1％and 88.5-111.0％with the relative standard deviation less than 9.3％,respectively.Comparing the proposed method with HPLC,the test results of above two methods show no significant difference.The proposed method obtains satisfactory experimental results,which is of significance for the practical detection of 1-NA and 2-NA in textiles.关键词
禁用芳香胺/同分异构体/恒基体同步荧光法/导数技术/快速检测Key words
Banned aromatic amines/Isomers/Matrix isopotential synchronous fluorescence spectroscopy/Deriva-tive technique/Rapid detection分类
化学化工引用本文复制引用
李娜,魏嘉雯,吴平平,赵妍,谢堂堂,李耀群..基于导数-恒基体同步荧光的纺织品中萘胺同分异构体快速定量检测[J].生命科学仪器,2023,21(5):31-37,7.基金项目
福建省科技计划项目(项目号:2022Y4008) （项目号:2022Y4008）
国家自然科学基金(项目号:22274137)资助 （项目号:22274137）
