山西农业大学学报（自然科学版）2024，Vol.44Issue(1)：1-13,13.DOI:10.13842/j.cnki.issn1671-8151.202311021
青蒿查尔酮合成酶(AaCHS)基因家族鉴定及光调控分析
Identification and photoregulation analysis of Artemisinin chalcone synthetase(AaCHS)gene family and analysis of light regulation in Artemisia annua L.
摘要
Abstract
[Objective]The aim of this study was to understand the molecular evolutionary characteristics of the chalcone syn-thase(CHS)gene family of Artemisia annua L.and its expression patterns in different tissues.[Methods]The CHS protein HMM model was downloaded from Pfam database,and CHS gene family members in A.annua L.genome were identified us-ing HUMMER 3.0,Blastp,and CD-search.Bioinformatic analyses of amino acid and nucleotide sequences were conducted using TBtools,EXPASy,CELLO v.2.5,MEGA 7.0,MEME,SOPMA,SWISSMODEL,and PlantCARE.The expres-sion analysis of AaCHS gene family members was performed using transcriptome data from eight different tissues.[Results]The results showed that there were 16 members in the AaCHS gene family in A.annua genome.Protein structure analysis re-vealed that the protein structure was unstable,categorizing them into four groups.Gene structure analysis indicated that all AaCHS gene had exons,with the number ranging from 2 to 4,and eight AaCHS genes lacked introns,with the number rang-ing from 0 to 2.Promoter cis-element analysis revealed a high abundance of photoresponsive and phytohormone responsive ele-ments.CD-search verified the presence of the Chal-sti-synt_N domain in each AaCHS protein.[Conclusion]Expression analy-sis of AaCHS family members showed different expression patterns in various tissues and under light treatment.GO enrich-ment analysis of biological processes and molecular functions revealed 80 enriched sequences in the biological process category and 19 sequences in the cellular component category,with the cytoplasm being the most enriched subcellular component(11 se-quences).It is speculated that AaCHS genes regulate the accumulation of flavonoids in the cytoplasm.Differential expression analysis under light conditions regulation suggested that AaCHS7 and AaCHS10 played a positive regulatory role in A.annua L.under the red light treatment.关键词
青蒿/CHS基因家族/光调控/基因表达/差异基因表达Key words
Artemisia annua L./CHS gene family/Photoregulation/Gene expression/Differential gene expression分类
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薛天源,何钰晴,夏忙,陈美珠,戴希刚,何思晓,朱洵,曾长立..青蒿查尔酮合成酶(AaCHS)基因家族鉴定及光调控分析[J].山西农业大学学报（自然科学版）,2024,44(1):1-13,13.基金项目
湖北省重点研发计划项目(2021BBA097 （）
2022BBA0064) （）
江汉大学一流学科建设重大专项资助计划(2023XKZ026) （2023XKZ026）
