山西农业大学学报（自然科学版）2024，Vol.44Issue(1)：34-42,9.DOI:10.13842/j.cnki.issn1671-8151.202308028
外源褪黑素对玫瑰高温胁迫的缓解效应
Alleviating effects of exogenous melatonin on rose under high temperature stress
摘要
Abstract
[Objective]High temperature stress has become a major abiotic stress factor restricting the growth and production of roses.Exploring the impact of exogenous melatonin on the heat tolerance of roses aimed to provide practical and theoretical foundations for addressing heat damage issues in rose cultivations and the genetic improvement of heat tolerance in roses.[Methods]In this study,the two year old potted'Purple Branch'rose seedling was used as experimental materials,and the ef-fects of folia spraying with 100 μmol·L-1 melatonin on the relevant physiological characteristics and heat shock protein gene ex-pression in rose leaves under 40℃ high-temperature stress was investigated.[Results]The results showed that high tempera-ture stress resulted in imbalances in rose leaf water content,damaged to the cell membrane system,inhibition of photosynthesis,secondary oxidative stress,and a significant inhibition of rose growth.However,after exogenous melatonin pretreatment,the relative electrical conductivity(REC)and malondialdehyde(MDA)content in rose leaves were significantly decreased under high-temperature stress.Relative water content(RWC)and chlorophyll content(SPDA)significantly increased,while the max-imum photochemical efficiency of photosystem Ⅱ(Fv/Fm)and net photosynthetic rate(Pn)significantly improved.proline(Pro)and soluble protein(SP)content increased significantly.The activities of superoxide dismutase(SOD),peroxidase(POD),and catalase(CAT)significantly enhanced.The expression levels of three heat shock protein genes,including RhH-SP70,RhHSP90 and RhHSP101,were significantly upregulated.[Conclusion]Under high-temperature stress,exogenous melatonin pretreatment can alleviate the adverse effects on rose leaves by enhancing water retention capacity,maintaining the stability and integrity of the cell membrane system,increasing photosynthetic intensity,raising osmoregulatory substance lev-els,boosting antioxidant enzyme activity,and upregulating the expression of heat shock protein genes.This ultimately enhance the heat tolerance of rose.关键词
褪黑素/玫瑰/高温胁迫/生理特性/热激蛋白Key words
Melatonin/Rose/High temperature stress/Physiological characteristics/Heat shock protein分类
农业科技引用本文复制引用
田雪军,徐佩琦,吴晶晶,徐艳,熊兴鹏..外源褪黑素对玫瑰高温胁迫的缓解效应[J].山西农业大学学报（自然科学版）,2024,44(1):34-42,9.基金项目
湖北省教育厅科研计划项目(Q20194304) （Q20194304）
特色花卉生物育种湖北省工程研究中心开放课题资助项目(2022ZD008) （2022ZD008）
荆楚理工学院大学生创新创业训练计划项目(KC2022027) （KC2022027）
