山西农业大学学报（自然科学版）2024，Vol.44Issue(1)：43-52,10.DOI:10.13842/j.cnki.issn1671-8151.202308025
影响'红满堂'绿枝插条生根因子的研究
Study on the factors influencing the rooting of green branch cuttings in'Hongmantang'variety
摘要
Abstract
[Objective]This study aimed to investigate the effect of cutting substrate,cutting position,growth regulators,and the duration of growth regulator treatment on the rooting efficiency of green branch cuttings of the'Hongmantang'and to identify suitable combination for green branch cuttings,providing theoretical and technical support for its factory seedling cultivation.[Method]The current year green branches of'Hongmantang'variety was used as research materials,and L9(34)orthogonal ex-perimental design was adopted.Starting from three days after cutting,observations of changes at the base of the cuttings were made every 3～4 days.Rooting conditions of the cuttings was recorded,and at 50 days post-cutting,the rooting rate,number of adventitious roots,average length of adventitious root,and the longest adventitious root length were investigated to compre-hensively evaluate the rooting effect.[Result]The dominant factor affecting the rooting of green branch cuttings in'Hongman-tang'variety was the cutting position.Cutting from the middle and lower parts of the same branch,which were relatively well-developed and lignified,exhibited significantly higher rooting rates at 36.67% and 40.67% than upper cuttings at 9.33% .The key factors affecting cutting propagation of'Hongmantang'were determined as the type,concentration,and treatment duration of growth regulator which displayed a significant impact on rooting rate,number of adventitious roots,average and longest ad-ventitious root,and comprehensive evaluation of rooting effect.Different growth regulators and treatment durations resulted in rooting rates ranging from 14.67% to 38.00% and 23.33% to 36.00%,adventitious root numbers of 2.40 to 5.03 and 3.40 to 4.57,average length of 1.93 to 2.27 and 1.10 to 2.93 cm,the longest length of 2.47 to 3.41 and 1.66 to 3.39 cm,and com-prehensive evaluation value of 43.70% to 86.40% and 56.00% to 81.00% for the'Hongmantang'green branch cuttings.The cutting substrate significantly affected the growth and development of adventitious root,with different substrates resulting in ad-ventitious root number of 3.37 to 5.00,average lengths of 1.34 to 3.10 cm,and comprehensive evaluation values of 58.43% to 76.37% .[Conclusion]The green branch cutting of'Hongmantang'belongs to the comprehensive rooting type,with a pre-dominant focus on callus tissue rooting.The optimal treatment combination for rooting green branch cutting involved using the lower part of the current year green branches,soaking the cutting base in IBA at a concentration of 2500 mg·L-1 for 20 s,and planting in a substrate mix of coconut coir,vermiculite,and perlite at 1∶2∶4.For optimal root development,using the middle part of the current year green branches,soaking the cutting base IBA at a concentration of 2500 mg·L-1 for 5 s,and planting in a substrate mix of peat,vermiculite,and perlite at 1∶2∶4 was recommended.关键词
苹果属/'红满堂'品种/绿枝扦插/影响因子/生根率Key words
Malus/'Hongmantang'/Green branch cutting/Effect factors/Rooting ratio分类
农业科技引用本文复制引用
杜学梅,高敬东,王骞,蔡华成,李春燕,王淑婷,杨廷桢..影响'红满堂'绿枝插条生根因子的研究[J].山西农业大学学报（自然科学版）,2024,44(1):43-52,10.基金项目
国家现代苹果产业技术体系晋中综合试验站(CARS-27) （CARS-27）
山西省科技重大专项计划课题(202201140601027-1) （202201140601027-1）
山西农业大学生物育种工程项目(YZGC034) （YZGC034）
