北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析OA北大核心CSTPCD

The study aimed to conduct an association analysis of GREB1L and MIB1 gene polymorphisms with rib number and carcass traits(carcass length,straight length,diagonal length and carcass weight),in order to screen for key functional loci.In this study,DNA was extracted from ear tissue of 317 healthy Beijing black pigs at the age of 215 days.The primers were designed for GREB1L and MIB1.The exon regions of two genes were genotyped by PCR and Sanger sequencing.The distribution of genotype frequency and allele frequency of the mutated loci was calculated,and their association analysis with rib number and carcass traits was performed.A total of 6 SNPs were detected in two genes,including 3 synonymous mutations in GREB1L,2 synonymous mutations in MIB1,one splice donor 5th base variant-intron variant in MIB1.Statistical analysis using Duncan's multiple test revealed that 3 synonymous mutations(6:106574972 G＞A,6:106648540 C＞T,6:106648558 A＞G)in GREB1L and one synonymous mutation(6:107028660 G＞A)in MIB1 were no significant association with traits(P＞0.05).A synonymous mutation in MIB1(6:106936590 C＞T)was significant association with carcass length and one splice donor 5th base variant-intron variant in MIB1(6:107016475 A＞G)was significant association with the number of rib(P＜0.05).In conclusion,the above two significant SNPs could be used as candidate functional loci for the variation of rib number and carcass length of Beijing black pigs.