畜牧兽医学报2024，Vol.55Issue(1)：79-86,8.DOI:10.11843/j.issn.0366-6964.2024.01.009
北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析
Association of Polymorphisms of GREB1L and MIB1 Genes with Rib Number and Carcass Traits in Beijing Black Pigs
摘要
Abstract
The study aimed to conduct an association analysis of GREB1L and MIB1 gene polymorphisms with rib number and carcass traits(carcass length,straight length,diagonal length and carcass weight),in order to screen for key functional loci.In this study,DNA was extracted from ear tissue of 317 healthy Beijing black pigs at the age of 215 days.The primers were designed for GREB1L and MIB1.The exon regions of two genes were genotyped by PCR and Sanger sequencing.The distribution of genotype frequency and allele frequency of the mutated loci was calculated,and their association analysis with rib number and carcass traits was performed.A total of 6 SNPs were detected in two genes,including 3 synonymous mutations in GREB1L,2 synonymous mutations in MIB1,one splice donor 5th base variant-intron variant in MIB1.Statistical analysis using Duncan's multiple test revealed that 3 synonymous mutations(6:106574972 G＞A,6:106648540 C＞T,6:106648558 A＞G)in GREB1L and one synonymous mutation(6:107028660 G＞A)in MIB1 were no significant association with traits(P＞0.05).A synonymous mutation in MIB1(6:106936590 C＞T)was significant association with carcass length and one splice donor 5th base variant-intron variant in MIB1(6:107016475 A＞G)was significant association with the number of rib(P＜0.05).In conclusion,the above two significant SNPs could be used as candidate functional loci for the variation of rib number and carcass length of Beijing black pigs.关键词
北京黑猪/GREB1L/MIB1/肋骨数/胴体性状Key words
Beijing black pigs/GREB1L/MIB1/rib number/carcass traits分类
农业科技引用本文复制引用
牛乃琪,赵润泽,宗文成,刘先策,刘海,石国华,井西涛,张龙超..北京黑猪GREB1L和MIB1基因多态性与肋骨数及胴体性状的关联分析[J].畜牧兽医学报,2024,55(1):79-86,8.基金项目
国家重点研发计划项目(2021YFD1301101) （2021YFD1301101）
国家生猪产业技术体系(CARS-35) （CARS-35）
中国农业科学院科技创新工程(ASTIP-IAS02) （ASTIP-IAS02）
