畜牧兽医学报2024，Vol.55Issue(1)：373-387,15.DOI:10.11843/j.issn.0366-6964.2024.01.035
奶牛产后急性子宫内膜炎血液氧化脂质组变化特征
Study on the Changes of Blood Oxidized Lipid Group in Postpartum Dairy Cows with Acute Endometritis
摘要
Abstract
The pathogenesis of acute Endometritis in postpartum dairy cows was elucidated from the change characteristics of blood lipid oxide group.Seven Holstein dairy cows with endometritis within 7 days after delivery,which were similar body condition scoring and tire postpartum parity,were selected from a large-scale intensive dairy farm in Ningxia.Before treatment,they were regarded as test group(E),and after treatment,they were regarded as control group(C).Blood samples were collected before morning feeding and plasma was prepared.The content of oxidized lipids in plasma was quantitatively detected by high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The changes of plasma oxidized lipid metabolic profiles were analyzed by lipomics technology,and the differentially oxidized lipid metabolites were screened for metabolic pathway enrichment analysis.The plasma metabolic profile of E group was significantly different from that of C group(P＜0.05,VIP≥ 1,FC ≥2 or FC≤0.5),of which 4 were up-regulated,including 12-HEPE(hydroxyeicosapentaenoic acid),5-HEPE,12-HETE(hydroxyeicosatetraenoic acid),14(S)-HDH A(hydroxydocosahexaenoic acid),and 6 were down-regulated,including 9,10-EpOME(epoxyoctadecenoic acid),12,13-EpOME,11,12-EET(epoxyeicosatrienoic acid),8,9-EET,14,15-EET and 17(18)-EpETE(epoxyeicosatetraenoic acid);The differential metabolites were enriched in arachidonic acid metabolism,inflammatory mediator regulation of TRP channel,PPAR signaling pathway,vascular smooth muscle contraction.The occurrence of acute endometritis in postpartum dairy cows is closely related to the changes of oxidized lipids in vivo.These oxidized lipids may participate in the occurrence and development of postpartum acute endometritis through arachidonic acid metabolic pathway,inflammatory mediator regulation of TRP channel,PPAR signaling pathway and vascular smooth muscle contraction.关键词
产后急性子宫内膜炎/氧化脂质/差异代谢物/代谢通路/发病机制Key words
endometritis/oxidized lipid/differential metabolites/metabolic pathway/pathogenesis分类
农业科技引用本文复制引用
王瑞玲,王雪妍,王菲菲,孔维怡,毛永霞,刘欣,丁辉,许立华,郭延生..奶牛产后急性子宫内膜炎血液氧化脂质组变化特征[J].畜牧兽医学报,2024,55(1):373-387,15.基金项目
国家自然科学基金(32160848) （32160848）
国家自然科学基金(31860719) （31860719）
宁夏奶牛育种专项(2019NYYZ05) （2019NYYZ05）
