畜牧兽医学报2024，Vol.55Issue(1)：401-405,5.DOI:10.11843/j.issn.0366-6964.2024.01.038
猫杯状病毒单克隆抗体的制备
Development of Monoclonal Antibodies against Feline Calicivirus
摘要
Abstract
This study aimed to develop specific monoclonal antibodies for feline calicivirus(FCV)and provide materials for immunological diagnostic methods of FCV.In this study,BALB/c mice were immunized with FCV purified by sucrose gradient centrifugation and inactivated.The positive hybridoma cells were screened by an indirect ELISA method and characterized by a series of methods.One hybridoma 3B11 stably secreting monoclonal antibodies was successfully obtained.The antibody type of MAb 3B11 was identified as IgG1 for the heavy chain and κ chain for the light chain.MAb 3B11 specifically reacted with FCV-SH192.The mouse ascites MAb 3B11 showed high ELISA titers against five FCV strains;it had IFA and Western blot binding characteristics with five FCV strains.The MAb 3B11 developed in this study has good specificity,immunoreactivity and broad-spectrum recognition of FCV,which provides biological raw materials for the optimization of FCV diagnostic methods,screening of epidemic strains and basic research.关键词
猫杯状病毒/单克隆抗体/特异性/反应性Key words
feline calicivirus/monoclonal antibodies/specificity/reactivity分类
农业科技引用本文复制引用
王莹,李佳康,曾悦,史开拓,韦丽婷,彭佳佳,李秋燕,曹龙龙,周登元..猫杯状病毒单克隆抗体的制备[J].畜牧兽医学报,2024,55(1):401-405,5.基金项目
武汉市"3551创新人才计划" （）
