大豆卵磷脂对湖羊精液4℃保存效果的影响OACSTPCD

In order to investigate the effect of soy lecithin(SL)on the preservation of Hu sheep semen at 4℃,different concentrations(0,0.1％,0.2％,0.3％and0.4％)of SL were added to the base dilution of Hu sheep semen for 4℃ cryopreservation test.The optimum concentration of SL in the cryopreservation diluent of Hu sheep semen was determined by measuring sperm motility,viability,linear rate,curve rate,path rate,plasma membrane intactness and acrosome intactness at different points of the preservation time(0-6 days).The re-sults showed that for 2,5 and 6 d of preservation,the sperm motility and vitality of the 0.1％ SL group were significantly higher than those of the other groups(P<0.05);for3 d of preservation,the linear rate of the 0.1％ SL group was significantly higher than those of the other groups(P<0.05);for 4 d of preservation,the curve rate and path rate of the SL groups were significantly higher than those of the control group(P<0.05);for 1 and 5 d of preservation,the sperm plasma membrane integrity rate was significantly higher in the 0.1％ SL group than in the other groups(P<0.05);and for 2 and 6 d of preservation,the sperm acrosome integrity rate was significantly higher in the 0.1％ SL group than in the other four groups(P<0.05).In conclusion,under cryopreservation conditions,the addition of 0.1％ SL effectively pro-longed the survival time of spermatozoa and improved the quality of semen in Hu sheep.