饲粮添加芦丁对断奶仔猪回肠形态结构和屏障功能的影响OACSTPCD

The aim of this study was to determine the effects of dietary rutin on the morphology,mucosal barrier,antioxidant function,in-flammation and apoptosis of the intestines in weaned piglets.Sixteen 28-day-old healthy weaned piglets with similar body weight were select-ed and randomly divided into 2 groups with 8 replicates per group and 1 pig per replicate.The piglets in the control group were fed with a bas-al diet,and those in the rutin group were fed with basal diet supplemented with 500 mg/kg rutin.The experiment lasted for 14 days.The re-sults showed as follow:Compared with the piglets of the control group,dietary 500 mg/kg rutin had no significant effect on the morphology of the ileum of the piglets of the rutin group(P＞0.05),but significantly increased the catalase(CAT)activity of their ileum mucosa(P＜0.05).The mRNA expression of B-cell lymphoma gen-2(Bcl-2),superoxide dismutase 1(SOD1),superoxide dismutase 2(SOD2)and occlusive small loop protein-1(ZO-1)in the ileal mucosa of the experimental piglets was significantly increased(P＜0.05),and the mR-NA and protein expression of Occludin in the piglets was significantly increased(P＜0.05)by the supplementary dietary rutin,while the mRNA expression levels of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in ileal mucosa of the piglets were significantly re-duced(P＜0.05).These results suggested that dietary 500 mg/kg rutin improved antioxidant capacity and intestinal barrier function of weaned piglets,and alleviated inflammation and apoptosis in them,which might be one of the important reasons for inhibiting diarrhea in weaned piglets by rutin.