常山酮和盐霉素协同抑制犬乳腺肿瘤细胞的增殖、迁移和克隆形成OACSTPCD

In this study,the effects of the combination of genitone and salinomycin on the proliferation,migration and clonal formation of canine breast tumor cells were evaluated using CMT-N7 isolated from canine breast tumor tissue as an in vitro model.The combination of halofuginone,salinomycin and two drugs was used to evaluate the inhibitory effect of the two drugs on canine breast tumor cells,the combina-tion index was calculated using the Chou-Talalay method to determine the optimal synergistic ratio,and scratch test and cloning formation test were further used to evaluate the effect of the combination of the two drugs on the migration and clonal formation abilities of canine breast tumor cells under the synergistic ratio.The results showed that the proliferation of CMT-N7 cells was inhibited in a concentration-dependent manner,the combination of the two drugs had a synergistic inhibitory effect,and the optimal synergistic ratio was 1:1.At this ratio,the combination of the two drugs had a more significant inhibitory effect on the migration and clonal formation of canine breast tumor cells than that of a single drug(P<0.01).Therefore,the combination of halofuginone and salinomycin synergistically inhibited the proliferation,migra-tion and clonal formation of canine breast tumor cells.This study might serve as a basis and reference for the combined application of veteri-nary clinical antitumor drugs in the future.