畜牧与兽医2024，Vol.56Issue(2)：31-36,6.
常山酮和盐霉素协同抑制犬乳腺肿瘤细胞的增殖、迁移和克隆形成
Halofuginone and salinomycin synergistically inhibit the proliferation,migration and clonal formation of canine mammary tumor cells
摘要
Abstract
In this study,the effects of the combination of genitone and salinomycin on the proliferation,migration and clonal formation of canine breast tumor cells were evaluated using CMT-N7 isolated from canine breast tumor tissue as an in vitro model.The combination of halofuginone,salinomycin and two drugs was used to evaluate the inhibitory effect of the two drugs on canine breast tumor cells,the combina-tion index was calculated using the Chou-Talalay method to determine the optimal synergistic ratio,and scratch test and cloning formation test were further used to evaluate the effect of the combination of the two drugs on the migration and clonal formation abilities of canine breast tumor cells under the synergistic ratio.The results showed that the proliferation of CMT-N7 cells was inhibited in a concentration-dependent manner,the combination of the two drugs had a synergistic inhibitory effect,and the optimal synergistic ratio was 1:1.At this ratio,the combination of the two drugs had a more significant inhibitory effect on the migration and clonal formation of canine breast tumor cells than that of a single drug(P<0.01).Therefore,the combination of halofuginone and salinomycin synergistically inhibited the proliferation,migra-tion and clonal formation of canine breast tumor cells.This study might serve as a basis and reference for the combined application of veteri-nary clinical antitumor drugs in the future.关键词
常山酮/盐霉素/协同抑制/犬乳腺肿瘤细胞Key words
halofuginone/salinomycin/synergistic inhibition/canine mammary tumor cell分类
农业科技引用本文复制引用
胡诗恒,江善祥,郭大伟,宋昕昊,林梦娟,郑玉玲,王俊棋,游凤,吕英军,高修歌,张军忍..常山酮和盐霉素协同抑制犬乳腺肿瘤细胞的增殖、迁移和克隆形成[J].畜牧与兽医,2024,56(2):31-36,6.基金项目
国家自然科学基金项目(32172918) （32172918）
中央高校基本科研业务费项目(KYGD202002) （KYGD202002）
