畜牧与兽医2024，Vol.56Issue(2)：104-109,6.
日本脑炎病毒感染睾丸间质细胞对干扰素调节因子3相关信号通路的影响
Effect of Japanese encephalitis virus infection on the IRF3 related signal pathway in leydig cells
摘要
Abstract
The aim of the study was to investigate the innate immune mechanism triggered by Japanese encephalitis virus(JEV)infection of testicular interstitial cells by activating pattern recognition receptors,such as Retinoic acid inducible gene I like receptors(RIG-Ⅰ)and Toll like receptor-3(TLR3).A model of JEV infection in testicular interstitial cells was constructed,and cellular lesions at different time periods after JEV infection were observed.Then,fluorescence quantitative PCR and Western Blot techniques were used to observe the tran-scription and protein expression of RIG-Ⅰ,TLR3,and IRF3 in cells at different infection stages,and the phosphorylation of IRF3 and the transcription status of IFN-β transcription status were detected.The results showed that,after JEV infection with testicular interstitial cells,the mRNA and protein expression levels of RIG-Ⅰand IRF3 were significantly increased(P＜0.05),the TLR3 protein expression level was significantly upregulated(P＜0.01),the IRF3 phosphorylation level was significantly upregulated(P＜0.001),and the transcription level of IFN-β significantly increased(P＜0.05).The results indicated that JEV infection activated the RIG-Ⅰand TLR3 receptors in the testicu-lar interstitial cells,thereby activating IRF3 phosphorylation and initiating the secretion of IFN-β,and triggered the innate immune antiviral immune response in the body.关键词
日本脑炎病毒/模式识别受体/干扰素调节因子3/先天免疫Key words
Japanese encephalitis virus/pattern recognition receptors/interferon regulatory factors 3/innate immunity分类
农业科技引用本文复制引用
陈阊峥,汤德元,罗柳,廖正波,袁盛林,陈旭..日本脑炎病毒感染睾丸间质细胞对干扰素调节因子3相关信号通路的影响[J].畜牧与兽医,2024,56(2):104-109,6.基金项目
贵州省科技支撑计划项目(黔科合支撑[2021]一般162) （黔科合支撑[2021]一般162）
贵州省科技支撑计划项目(黔科合支撑[2018]2265) （黔科合支撑[2018]2265）
