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An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Zhongyi ZHANG Hang LIU Xiaohui PAN Yanan ZONG Leili FENG Lixian LIU Li GUO Guanpin YANG

海洋湖沼学报（英文版）2024，Vol.42Issue(1)：216-225,10.

海洋湖沼学报（英文版）2024，Vol.42Issue(1)：216-225,10.DOI:10.1007/s00343-023-2332-1

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Zhongyi ZHANG ¹Hang LIU ¹Xiaohui PAN ¹Yanan ZONG ¹Leili FENG ¹Lixian LIU ¹Li GUO ¹Guanpin YANG²

作者信息

1. College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China
2. College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China||Institutes of Evolution and Marine Biodiversity,Ocean University of China,Qingdao 266003,China||Key Laboratory of Marine Genetics and Breeding of Ministry of Education,Ocean University of China,Qingdao 266003,China
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摘要

关键词

Nannochloropsis oceanica/genetic transformation/random insertional mutant library/zeocin pretreatment/forward genetics

Key words

Nannochloropsis oceanica/genetic transformation/random insertional mutant library/zeocin pretreatment/forward genetics

引用本文复制引用

Zhongyi ZHANG,Hang LIU,Xiaohui PAN,Yanan ZONG,Leili FENG,Lixian LIU,Li GUO,Guanpin YANG..An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica[J].海洋湖沼学报（英文版）,2024,42(1):216-225,10.

基金项目

Supported by the National Key R&D Program of China(Nos.2018YFD0901506,2018YFD0900305)and the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018 SDKJ0406-3) （Nos.2018YFD0901506,2018YFD0900305）

海洋湖沼学报（英文版）

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ISSN：2096-5508

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