中国计量大学学报2023，Vol.34Issue(4)：626-636,11.DOI:10.3969/j.issn.2096-2835.2023.04.018
乌牛早黑茶多糖及其自由基降解产物体外抗氧化和降脂活性研究
Evaluation of the in vitro antioxidant and lipid-lowering activity of Wuniuzao dark tea polysaccharides and its free radical degradation products
摘要
Abstract
Aims:The physical and chemical properties and in vitro functional activities of Wuniuzao dark tea polysaccharides(WDTP)and its degradation products were investigated.Methods:WDTP was prepared by the hot water extraction method,and its degradation products were obtained by the free radical degradation method.The physical and chemical properties of WDTP and its degradation products were analyzed by ultraviolet spectroscopy,high performance liquid chromatography and high performance gel chromatography.The antioxidant activity of WDTP and its degradation products in vitro was compared by the DPPH free radical scavenging assay,the ABTS free radical scavenging assay,the hydroxyl free radical scavenging assay and the potassium ferricyanide method.The lipid-lowering activity of the WDTP and its degradation products in vitro was compared by the pancreatic lipase inhibition assay and the lipid accumulation assay in HepG 2 cells.Results:After free radical degradation,the content of WDTP increased from 63.74％to 65.28％.The content of uronic acid decreased from 17.15％to 14.67％.The composition of monosaccharides was the same.The molar ratio of monosaccharides was slightly different;and the weight average molecular weight of WDTP decreased from 67 303 u to 55 423 u.Compared with WDTP before degradation,the scavenging activities of DPPH radical,ABTS radical,hydroxyl radical and the total reducing power of WDTP degradation products were significantly enhanced.WDTP degradation products inhibited pancreatic lipase activity and lipid accumulation in HepG2 cells by increasing the mRNA expression levels of PPAR-a,CPT-1A and CYP7A1 genes.Conclusions:WDTP has changed its physical and chemical properties after free radical degradation;and its antioxidant and lipid-lowering activities are enhanced in vitro.关键词
乌牛早黑茶多糖/自由基降解/理化性质/抗氧化/降脂Key words
Wuniuzao dark tea polysaccharides/free radical degradation/antioxidant activity/lipid-lowering activity分类
医药卫生引用本文复制引用
董宗华,邹旋旋,常帅帅,叶露婷,陈昭闻,赵进,葛建..乌牛早黑茶多糖及其自由基降解产物体外抗氧化和降脂活性研究[J].中国计量大学学报,2023,34(4):626-636,11.基金项目
国家自然科学基金项目(No.31100499),浙江省重大科技专项项目(No.2020C02045) （No.31100499）
